MutaGEL® Oxidative Stress I PCR Assay

$750.00

The Eagle Biosciences MutaGEL® Oxstress I PCR Assay kit allows detection of the genotypes T-786C (promoter region) of the endothelial NO Synthase gene (NOS3) and C242T (His72Tyr) of the NAD(P)H Oxidase subunit CYBA in human genomic DNA probes. The Eagle Biosciences MutaGEL® Oxstress I is for research use only and is not intended for diagnostic or therapeutic procedures.

SKU: KE09008 Categories: ,

MutaGEL® Oxidative Stress I PCR Assay

For Research Use Only

Size: 24 Samples
Method: PCR (RFLP)
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL

Product manufactured in Germany by Immundiagnostik

Additional Information

Assay Background

Within the metabolic status named “oxidative stress,” the production of molecules with free reactive electrons from e.g. glycolysis is not equaled by its normal potent lipidic or enzymatic electron acceptors. This causes diabetes, cancer and aging. The levels of several indicative molecules vary under the influence of some common DNA polymorphisms of enzymes, which metabolize the agonists of the radicalic or nonradicalic oxidants – mainly superoxide and hydroxide anion – such as T-786C of the NOSynthase promotor, C242T (His72Tyr) in a subunit of the NAD(P)H oxidase macromolecule. Additionally, the common complete deletion of glutathion-S-transferase genes M1 and T1 (see “MutaGEL® GST-M1/T1”), as well as sequence variation of superoxide-dismutase SOD2 and catalase (see “MutaGEL® Oxstress II”), influence the radicalic situation in the body.

Assay Principle

With the Eagle Biosciences MutaGEL® Oxstress I two DNA regions specific for the critical gene parts of eNOS and NAD(P)H Oxidase are amplified in parallel reactions. The amplification products are then treated with a mix of restriction enzymes, which are sensitive for the “negative” sequence variant of each gene – in parallel as well – such that in the following gel the genotype of the probe can be recognised for both polymorphisms from the length of the resulting restriction fragments (RFLP method).

Manual

Product Manual