MutaGEL Lactase PCR Assay

$775.00

The MutaGEL® Lactase (AS) kit allows the detection of the common -13910 T/C polymorphism in the lactase gene by direct allele-specific detection of genotype. The Eagle Biosciences MutaGEL® Lactase (AS) is for research use only and is not intended for diagnostic or therapeutic procedures.

SKU: KE09009 Categories: ,

MutaGEL Lactase (AS) PCR Assay

MutaGEL Lactase PCR Assay manufactured in Germany by Immundiagnostik

Size: 24 Samples
Method: PCR (allele specific)
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL
For Research Use Only
Controls Included


Storage and Stability
Store at ≤ -18°C. The reagents are stable in the unopened micro tubes until the expiration date indicated (see print on the package). Don’t thaw out the content of the “positive control DNA” for more than two times. If necessary, make suitable aliquots. Before use: Spin tubes briefly before opening (contents may become dispersed during shipment).


Assay Principle

The MutaGEL Lactase PCR Assay is an amplification refractionary mutation system (ARMS) containing two sets of primers for allele-specific sequences within the lactase gene. The sequence-specific primers can be used directly for PCR with extracted DNA. The resulting amplification products are subsequently identified with gelelectrophoretic methods. If there is no specific allele-product detectable, the correct PCR procedure is proved by an internal control. The present genotype of unknown samples is interpreted by detection of corresponding DNA-amplificates for wildtype- and mutation-constellation in two separate lanes of the gel (method by Dr. Essrich, Biologisches Labor, Denzlingen).


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Additional Information

Assay Background


Patients with lactose intolerance are not able to digest milk sugar taken in with the food. Due to this fact, these persons suffer subsequently under malabsorption problems like nausea, flatulence, diarrhoea or stomach pain. The most important reason for lactose intolerance is founded in a genetically caused lack of the enzyme lactase which is responsible for the degradation of milk sugar in the organism. This common gene defect is very easy to detect by analysing the T/C base replacement at position -13910 from the regulatory region of lactase gene (LCT). If this base pair exchange from T to C is homozygous, a lactase-deficiency and subsequently lactose intolerance is predetermined. The manifestation of the disease occurs with about 20 years and the prevalence of the homozygous C / C – genotype in Germany is about 15 %, in Mediterranean countries up to 50%.

Manual

Product Manual