MutaGEL GST-P1 PCR Assay Kit

$775.00

The MutaGEL® GST-P1 PCR Assay allows the detection of the Ile105Val polymorphism in the human glutathione-S-transferase gene (GST-P1). The MutaGEL® GST-P1 PCR Assay is for research use only and is not intended for diagnostic or therapeutic procedures.

SKU: KV0903100 Categories: ,

MutaGEL GST-P1 PCR Assay Kit

MutaGEL GST-P1 PCR Assay manufactured in Germany by Immundiagnostik

Size: 24 Samples
Method: PCR (allele specific)
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL
For Research Use Only
Control Included


Assay Principle

The Eagle Biosciences MutaGEL GST-P1 PCR Assay kit contains a set of primers which amplify a specific sequence within the human GST-P1 gene (primer GST-P1). The amplified product obtained from a wild-type DNA will not be cut by the restriction enzyme included in this kit, whereas the fragment obtained from DNA carrying the Il105Val-mutation will be cut once. The identification of the present genotype is done by analysis of the amplification products and their cut fragments through gel electrophoresis.


Storage and Stability
Store at ≤ -18°C. The reagents are stable in the unopened micro tubes until the expiration date indicated (see print on the package). Don’t thaw out the content of the “GST-P1 positive control DNA” for more than three times. If necessary, make suiteable aliquots. Before use: Spin tubes briefly before opening (contents may become dispersed during shipment).


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Restrictions
The PCR results for all positive controls in DNA fragments of indicated length and for samples at least in the amplification product of 180 bp. If this is not the case, the sample must be tested a second time or the complete analysis must be repeated with freshly isolated DNA. If there are no positive control DNA fragments present, the amplification was incorrect and the chosen PCR conditions have to been proven/ corrected.

Additional Information

Assay Background


The glutathione-S-transferase system protects against peroxides and electrophilic reaction partners which both can damage the cells of the body. The enzyme exists in several different isoforms. The solubility of the (manifold) substrates is increased by the addition of glutathione leading to a better elimination of the toxic metabolites from the body. Glutathione-S-transferase deficiency can be genetically determined. The GST-P1 gene compensates (at least in parts) for the frequent deletion of GST-M1 or GST-T1 genes in Caucasians (about 50% respectively 20%).

Manual

Product Manual