MutaGEL® GST-P1 PCR Assay Kit

$750.00

The Eagle Biosciences MutaGEL® GST-P1 PCR Assay allows the detection of the Ile105Val polymorphism in the human glutathione-S-transferase gene (GST-P1). The MutaGEL® GST-P1 PCR Assay is for research use only and is not intended for diagnostic or therapeutic procedures.

SKU: KV0903100 Categories: ,

MutaGEL® GST-P1 PCR Assay Kit

For Research Use Only

Size: 24 Samples
Method: PCR (allele specific)
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL

Product manufactured in Germany by Immundiagnostik

Additional Information

Assay Background

The glutathione-S-transferase system protects against peroxides and electrophilic reaction partners which both can damage the cells of the body. The enzyme exists in several different isoforms. The solubility of the (manifold) substrates is increased by the addition of glutathione leading to a better elimination of the toxic metabolites from the body. Glutathione-S-transferase deficiency can be genetically determined. The GST-P1 gene compensates (at least in parts) for the frequent deletion of GST-M1 or GST-T1 genes in Caucasians (about 50% respectively 20%).

Assay Principle

The Eagle Biosciences MutaGEL GST-P1 PCR Assay kit contains a set of primers which amplify a specific sequence within the human GST-P1 gene (primer GST-P1). The amplified product obtained from a wild-type DNA will not be cut by the restriction enzyme included in this kit, whereas the fragment obtained from DNA carrying the Il105Val-mutation will be cut once. The identification of the present genotype is done by analysis of the amplification products and their cut fragments through gel electrophoresis.

Manual

Product Manual