MutaGEL® Apolipoprotein B 100 PCR Assay


The Eagle Biosciences MutaGEL® ApoB100 (Codon 3500) is a RFLP-coupled PCR test kit for the inspection of codon 3500 of the human apolipoprotein-B gene, with the purpose to detect DNA base G (normal) or A (mutant), causative for translated arginine or glutamine at this position in the ApoB100 protein. The Eagle Biosciences MutaGEL® ApoB100 is for research use only and is not intended for diagnostic or therapeutic procedures.

SKU: KE09023 Categories: ,

MutaGEL® Apolipoprotein B 100 PCR Assay Kit

For Research Use Only

Size: 24 Samples
Method: PCR (allel specific)
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL

Product manufactured in Germany by Immundiagnostik

Additional Information

Assay Background

Familial Hypercholesterolemia (FH) is a frequent cause of coronary artery disease in human beings. The normal removal of cholesterine from blood is interrupted, when DNA mutations of its liver receptor r-LDL make it defective or, as was found later, if the cholesterine carrier ApoB100 has gene mutations at or around codon 3500. Cd. 3500 Arg-Glu (G-A) is a mutation of Central European abundance and on average 1 of 250 persons is heterozygous for the dominant trait. The pathogenicity of the defective protein varies with further social and unknown genetic mutations: At age 50 20% of female mutation carriers have severe coronary artery disease and 40% of males. FH is easier screened for mutations of ApoB100 than for mutations of the LDL receptor gene.

Assay Principle

The Eagle Biosciences 24-sample kit’s PCR mix a piece of DNA sequence around codon 3500 can be amplified and later on incubated at elevated temperature with a bacterial restriction enzyme, which can recognize and cut the amplimere, when an A-mutation is present and not in the case of a normal G. The effect of the restriction experiment can be controlled by comparing the length of uncut and cut fragments to DNA of known size with a fluorescently coloured electrophoresis gel and UV light. Copyright: the intellectual property for this method owns to Dr.M.Eßrich, Denzlingen/Freiburg (Germany).


Product Manual