Mouse TNF-Alpha ELISA Assay Kit

$460.00

The Eagle Biosciences Mouse TNF-Alpha ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring TNF-a in biological samples of mice.

Mouse TNF-Alpha ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Dynamic Range: 31.2 pg/ml – 2000 pg/ml
Incubation Time: 3 hours 10 minutes
Sample Type: Plasma, Serum, Tissue Culture Fluids, Urine
Sample Size: 100 µl

Product manufactured in the USA

Additional Information

Assay Principle


The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the TNF-a present in samples reacts with the anti-TNF-a antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-TNF-a is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of TNF-a in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of TNF-a in the test sample. The quantity of TNF-a in the mouse sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Typical Standard Curve


Manual

Product Manual