Mouse TNF-Alpha ELISA Assay


The Mouse TNF-Alpha ELISA Assay is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring TNF-a in biological samples of mice.

Mouse TNF-Alpha ELISA Assay

The Mouse TNF-Alpha ELISA Assay is For Research Use Only

Size: 1×96 wells
Dynamic Range: 31.2 pg/ml – 2000 pg/ml
Incubation Time: 3 hours 10 minutes
Sample Type: Plasma, Serum, Tissue Culture Fluids, Urine
Sample Size: 100 µl

Assay Principle

In this Mouse TNF-Alpha ELISA Assay the TNF-a present in samples reacts with the anti-TNF-a antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-TNF-a is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of TNF-a in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of TNF-a. The quantity of TNF-a in the mouse sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze-thaw cycles.

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Additional Information

Typical Standard Curve

Mouse TNF-Alpha ELISA Assay


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