Neurology ELISA Assay

Mouse / Rat Noradrenaline ELISA

$890.00

The Mouse / Rat Noradrenaline (Norepinephrine) ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of noradrenaline in biological samples including serum, plasma, tissue, and cell culture samples.  The Mouse / Rat Noradrenaline (Norepinephrine) ELISA Assay kit is for research use only and should not be used in diagnostic procedures.

SKU: NOU39-K01 0 Categories: , , ,

Mouse / Rat Noradrenaline ELISA

Mouse Rat Noradrenaline ELISA manufactured in Germany as SKU EA633/96 by DLD Diagnostika

Size: 1×96 wells
Sensitivity: 1.0 pg/mL
Dynamic Range: 0.05 – 25 ng/ml
Incubation Time: Overnight
Sample Type: Biological Samples
Sample Size: 20 µL
Alternative Names: Mouse/Rat Norepinephrine ELISA Kit, Rodent Norepinephrine ELISA Kit, Rodent Noradrenaline Kit, Noradrenaline Sensitive ELISA
For Research Use Only
Controls Included

Conversion into pmol/l: Noradrenaline: 1 pg / ml = 5.91 pmol/l

Assay Background

The Noradrenaline (Norepinephrine) Mouse / Rat ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of noradrenaline in biological samples including serum, plasma, tissue, and cell culture samples.  The Rodent Noradrenaline (Norepinephrine) ELISA Assay kit is for research use only and should not be used in diagnostic procedures.  Catecholamine is the name of a group of aromatic amines (noradrenaline, adrenaline, dopamine, and their derivatives) which act as hormones and neurotransmitter, respectively. Adrenaline and noradrenaline are formed from dopamine. They act on the cardiac musculature and the metabolism (adrenaline) as well as on the peripheral circulation (noradrenaline) and help the body to cope with acute and chronic stress.

An increased production of catecholamines can be found with tumours of the chromaffine system (pheochromocytoma, neuroblastoma, ganglio­neuroma). An increased or decreased concentration of the catechol­amines can also be found with hypertension, degenerative cardiac diseases, schizophrenia and manic-depressive psychosis. The Rodent Noradrenaline (Norepinephrine) ELISA assay kit provides materials for the quantitative measurement of noradrenaline in low concentrated samples and for small sample volumes. Noradrenaline is extracted using a cis-diol-specific affinity gel and acylated to N-acylnoradrenaline and then converted enzymatically into N-acylnormetanephrine.

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Additional Information

Assay Principle

The competitive Mouse / Rat Noradrenaline (Norepinephrine) ELISA Assay kit uses the microtiter plate format. Noradrenaline is  bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.

Typical Standard Curve

Mouse Rat Noradrenaline ELISA

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Publications

Moustafa Algamal, Nicole Saltiel, Andrew J. Pearson, Bryan Ager, Ioana Burca, Benoit Mouzon, David M. Diamond, Michael Mullan, Joseph O. Ojo, and Fiona Crawford.Journal of Neurotrauma.http://doi.org/10.1089/neu.2018.6314

Raikwar, NS;Braverman, C;Snyder, PM;Fenton, RA;Meyerholz, DK;Abboud, FM;Harwani, SC; (2019). Am. J. Physiol. Heart Circ. Physiol.. doi:10.1152/ajpheart.00234.2019.

Citations

Goldstein N, McKnight AD, Carty JRE, et al. Hypothalamic Detection of Macronutrients Via Multiple Gut-Brain Pathways. Cell Metab. 2021;33:676-687.

Gargano, A., Beins, E., Zimmer, A., Bilkei-Gorzo, A. Lack of Cannabinoid Receptor Type-1 Leads to Enhanced Age-Related Neuronal Loss in the Locus Coeruleus. Int. J. Mol. Sci. (2021) https://doi.org/10.3390/ijms22010005

Riera, CE;Tsaousidou, E;Halloran, J;Follett, P;Hahn, O;Pereira, MMA;Ruud, LE;Alber, J;Tharp, K;Anderson, CM;Brönneke, H;Hampel, B;Filho, CDM;Stahl, A;Brüning, JC;Dillin, A;, (2017). The Sense of Smell Impacts Metabolic Health and Obesity. Cell Metab.(1), 198-211.e5-

Harwani, SC;Ratcliff, J;Sutterwala, FS;Ballas, ZK;Meyerholz, DK;Chapleau, MW;Abboud, FM;, Nicotine Mediates CD161a+ Renal Macrophage Infiltration and Premature Hypertension in the Spontaneously Hypertensive RatNovelty and Significance. Circulation Research, 1191101-1115.

Kandil, EA;Abdelkader, NF;El-Sayeh, BM;Saleh, S;, (2016). Imipramine and amitriptyline ameliorate the rotenone model of Parkinson’s disease in rats. Neuroscience, 33226-37.

Harwani, SC;Ratcliff, J;Sutterwala, FS;Ballas, ZK;Meyerholz, DK;Chapleau, MW;Abboud, F;, (2016). Nicotine Mediates CD161a+ Renal Macrophage Infiltration and Premature Hypertension in the Spontaneously Hypertensive Rat. Circ. Res..

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