Mouse/Rat Dopamine ELISA

$890.00

The Mouse/Rat Dopamine ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of dopamine in biological samples including serum, plasma, tissue, and cell culture samples. The Mouse/Rat Dopamine ELISA Assay kit is for research use only and should not be used in diagnostic procedures.

Mouse/Rat Dopamine ELISA

Mouse Rat Dopamine ELISA manufactured in Germany as SKU EA634/96 by DLD Diagnostika

Size: 1×96 wells
Sensitivity: 5.9 pg/mL
Dynamic Range: 0.3 – 100 pg/ml
Incubation Time: Overnight
Sample Type: Biological Samples
Sample Size: 20 µL
Alternative Names: Rodent Dopamine ELISA
For Research Use Only

Controls Included


Assay Principle

The Mouse / Rat Dopamine Assay Kit provides materials for the quantitative measurement of dopamine in low concentrated samples and for small sample volumes. In the Mouse/Rat Dopamine Assay, dopamine is extracted using a cis-diol-specific affinity gel and acylated to N-acyldopamine and then converted enzymatically into N-acyl-3-methoxytyramine. The Mouse/Rat Dopamine ELISA kit uses the microtiter plate format. Dopamine is bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.


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Dopamine ELISA Assay Kit

Additional Information

Assay Background


Catecholamine is the name of a group of aromatic amines (noradrenaline, adrenaline, dopamine, and their derivatives) which act as hormones and neurotransmitter, respectively. Adrenaline and noradrenaline are formed from dopamine. They act on the cardiac musculature and the metabolism (adrenaline) as well as on the peripheral circulation (noradrenaline) and help the body to cope with acute and chronic stress.
An increased production of catecholamines can be found with tumours of the chromaffine system (pheochromocytoma, neuroblastoma, ganglioneuroma). An increased or decreased concentration of the catecholamines can also be found with hypertension, degenerative cardiac diseases, schizophrenia and manic-depressive psychosis.

Typical Standard Curve


Mouse Rat Dopamine ELISA

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Citations


Master, ZR;Porzionato, A;Kesavan, K;Mason, A;Chavez-Valdez, R;Shirahata, M;Gauda, EB;, (2016). Lipopolysaccharide exposure during the early postnatal period adversely affects the structure and function of the developing rat carotid body, 121(3), 816-827. Kandil, EA;Abdelkader, NF;El-Sayeh, BM;Saleh, S;, (2016). Imipramine and amitriptyline ameliorate the rotenone model of Parkinson’s disease in rats. Neuroscience, 33226-37. Go, Ga-Yeon et al”Neuroprotective Effects of Hyangsayangwi-tang in MPTP-induced Mouse Model of Parkinson’s Disease Journal of Sasang Constitutional Medicine. 2014. Jun, 26(2): 165-179. Hou, S;Carson, DM;Wu, D;Klaw, MC;Houlé, JD;Tom, VJ. Dopamine is produced in the rat spinal cord and regulates micturition reflex after spinal cord injury.  Experimental Neurology.  2015.  doi:10.1016/j.expneurol.2015.12.001.