Mouse / Rat Dopamine ELISA Assay Kit

$870.00

The Mouse / Rat Dopamine ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of dopamine in biological samples including serum, plasma, tissue, and cell culture samples. The Mouse / Rat Dopamine ELISA Assay kit is for research use only and should not be used in diagnostic procedures.

Mouse / Rat Dopamine ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 5.9 pg/mL
Dynamic Range: 0.3 – 100 pg/ml
Incubation Time: Overnight
Sample Type: Biological Samples
Sample Size: 20 µL

Controls Included

Product Developed and Manufactured in Germany EA634/96

Additional Information

Assay Principle


The competitive Mouse / Rat Dopamine ELISA Assay kit uses the microtiter plate format. Dopamine is bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.

Typical Standard Curve


Manual

Product Manual


Publications

Citations


Master, ZR;Porzionato, A;Kesavan, K;Mason, A;Chavez-Valdez, R;Shirahata, M;Gauda, EB;, (2016). Lipopolysaccharide exposure during the early postnatal period adversely affects the structure and function of the developing rat carotid body, 121(3), 816-827. Kandil, EA;Abdelkader, NF;El-Sayeh, BM;Saleh, S;, (2016). Imipramine and amitriptyline ameliorate the rotenone model of Parkinson’s disease in rats. Neuroscience, 33226-37. Go, Ga-Yeon et al”Neuroprotective Effects of Hyangsayangwi-tang in MPTP-induced Mouse Model of Parkinson’s Disease Journal of Sasang Constitutional Medicine. 2014. Jun, 26(2): 165-179. Hou, S;Carson, DM;Wu, D;Klaw, MC;Houlé, JD;Tom, VJ. Dopamine is produced in the rat spinal cord and regulates micturition reflex after spinal cord injury.  Experimental Neurology.  2015.  doi:10.1016/j.expneurol.2015.12.001.