Mouse Rat ADMA ELISA Assay Kit

$1,460.00

The Eagle Biosciences Mouse/Rat ADMA ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of ADMA (Asymmetric Dimethylarginine) in human/mouse/rat plasma and serum samples as well as cell culture samples. The Mouse/Rat ADMA ELISA Assay kit is for research use only and should not be used in diagnostic procedures.

Mouse Rat ADMA ELISA

Mouse Rat ADMA ELISA Developed and Manufactured in Germany by DLD Diagnostika EA209/96

Size: 1×96 wells
Sensitivity: 0.01 µmol/l
Dynamic Range: 0.01 – 5 µmol/l
Incubation Time: Overnight
Sample Type: Serum, Plasma, Cell Culture
Species: Mouse, Rat
Sample Size: 20 µL
Alternative Names: Mouse Rat Asymmetric Dimethylarginine ELISA, Rodent ADMA ELISA
For Research Use Only

Controls Included


Assay Background


The vascular endothelium plays a central role in the regulation of vascular structure and function, mainly due to the formation of endothelium-derived nitric oxide (NO). NO has been named an “endogenous anti-atherogenic molecule” due to its diverse regulatory functions in vascular homeostasis. NO is formed by the enzyme NO synthetase (NOS) from the amino acid precursor L-arginine. NOS activity can be down-regulated by asymmetric dimethylarginine (ADMA), an endogenous inhibitor of NOS.

The effects of ADMA on NO synthesis and NO-mediated pathophysiological processes have been described in numerous experimental studies. Moreover, elevated ADMA levels in plasma have been found in clinical studies including patients with hypercholesterolemia, hypertension, chronic heart failure, chronic renal failure and other internal disorders. Recent prospective and cross-sectional studies indicated that elevated ADMA levels are a risk factor for future cardiovascular events and total mortality. ADMA may have diagnostic relevance as a novel cardiovascular risk marker.


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Related News

For a Full Listing of Publications utilizing these ADMA ELISA Assay kits, click on the link below:
ADMA ELISA Kit Publications

Learn more about ADMA at Eagle Biosciences Biomarker Spotlight page dedicated to ADMA here:
ADMA Biomarker Spotlight

Additional Information

Assay Principle


The new competitive Mouse/Rat ADMA ELISA uses the microtiter plate format. ADMA is bound to the solid phase of the microtiter plate. ADMA in the samples is acylated and competes with solid phase bound ADMA for a fixed number of rabbit anti-ADMA antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase ADMA is detected by anti-rabbit/peroxidase. The substrate TMB/peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase ADMA is inversely proportional to the ADMA concentration of the sample.

Assay Procedure


  1. Prepare all reagents, standard curve and samples as directed in the previous section.
  2. Pipette 50 µl of samples, standards or diluent (as negative control) into the wells.
  3. Immediately add 50 µl of HRP conjugate to each well.
  4. Mix gently.
  5. Seal wells with adhesive strip and incubate for 2 hours at room temperature.
  6. Aspirate fluid from wells and wash three times with 300 µl wash buffer. After the last wash, invert the plate and tap on a clean paper towel.
  7. Dispense 100 µl of TMB substrate solution into each well.
  8. Incubate for 20 minutes at room temperature in the dark.
  9. Add 100 µl of stop solution to each well.
  10. Determine the absorbance within 30 minutes at 450 nm. A reference wavelength of 620 nm/690 nm is recommended.

Typical Standard Curve


Mouse Rat ADMA ELISA

Manual

Product Manual


Publications

Citations


Baltgalvis, KA;White, K;Li, W;Claypool, MD;Lang, W;Alcantara, R;Singh, BK;Friera, AM;McLaughlin, J;Hansen, D;McCaughey, K;Nguyen, H;Smith, IJ;Godinez, G;Shaw, SJ;Goff, D;Singh, R;Markovtsov, V;Sun, TQ;Jenkins, Y;Uy, G;Li, Y;Pan, A;Gururaja, T;Lau, D;Park, Exercise performance and peripheral vascular insufficiency improve with AMPK activation in high-fat diet-fed mice, Am. J. Physiol. Heart Circ. Physiol., 2014, Volume 36, Issue 8, page H1128-45

Schulze F, Wesemann R, Schwedhelm E, Sydow K, Albsmeier J, Cooke JP, Böger RH. Determination of ADMA using a novel ELISA assay. Clin. Chem. Lab. Med. 2004; 42: 1377-1383

Krempl TK, Kähler J, Maas R, Silberhorn L, Meinertz T, Böger RH. Elevation of asymmetric dimethylarginine (ADMA) in patients with unstable angina and recurrent cardiovascular events. Eur. Heart J. 2005; 26: 1846-1851

Schulze F, Maas R, Freese R, Schwedhelm E, Silberhorn L, Böger RH. Determination of a reference value for N,N-dimethyl-L-arginine in 500 subjects. Eur. J. Clin. Invest. 2005; 35 : 622-626

Schnabel R, Blankenberg S, Lubos E, Lackner KJ, Rupprecht HJ, Espinola-Klein C, Jachmann N, Post F, Peetz D, Bickel C, Cambien F, Tiret L, Münzel T. Asymmetric dimethylarginine and the risk of cardiovascular events and death in patients with coronary artery disease: results from the AtheroGene Study. Circ. Res. 2005; 97: e53-59

O`Dwyer MJ, Dempsey F, Crowley V, Kelleher D, McManus R, Ryan T. Septic shock correlates with ADMA levels which may be influenced by a polymorphism in DDAH II: a prospective observational study. Crit. Care 2006; 10: (5): R139

Antoniades C, Tousoulis D, Marinou K, Vasiliadou C, Tentolouris C, Bouras G, Pitsavos C, Stefanidis C. Asymmetrical dimethylarginine regulates endothelial function in methionine-induced but not in chronic homocystinemia in humans: effect of oxidative stress and proinflammatory cytokines. Am. J. Clin. Nutr. 2006; 84: 781-788

Wang TZ., Chen WJ., Cheng WC., Lin JW., Chen MF., Lee YT. Relation of improvement in endothelium-dependent flowmediated vasodilation after Rosiglitazone to changes in asymmetric dimethylarginine, endothelin-1, and C-reactive protein in nondiabetic patients with the metabolic syndrome. Am. J. Cardiol. 2006; 9: 1057-1062

Wanby P., Nilsson I., Brudin L., Nyhammar I., Gustafsson I., Carlsson M. Increased plasma levels of asymmetric dimethylarginine in patients with carotid stenosis: no evidence for the role of the common FABBP2 A54T gene polymorphism. Acta Neurol. Scand. 2007; 115: 90-96

Konishi H, Sydow K, Cooke JP. Dimethylarginine dimethylaminohydrolase promotes endothelial repair after vascular injury. J. Am. Coll. Cardiol. 2007; 49: 1099-1105

Iribarren C, Husson G, Sydow K, Wang BY, Sidney S, Cooke JP. Asymmetric dimethyl-arginine and coronary artery calcification in young adults entering middle age: the CARDIA Study. Eur. J. Cardiovasc. Prev. Rehabil. 2007; 14:222-229

Melikian N, Wheatcroft SB, Ogah OS, Murphy C, Chowienczyk PJ, Wierzbicki AS, Sanders TA, Jiang B, Duncan ER, Shah AM, Kearney MT. Asymmetric dimethylarginine and reduced nitric oxide bioavailability in young Black African men Hypertension 2007; 49: 873-877

Horowitz JD, Heresztyn T. An overview of plasma concentrations of asymmetric dimethylarginine (ADMA) in health and disease and in clinical studies: Methodological considerations. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2007

Charitidou C, Farmakiotis D, Zournatzi V, Pidonia I, Pegiou T, Karamanis N, Hatzistilianou M, Katsikis I, Panidis D.The administration of estrogens, combined with anti-androgens, has beneficial effects on the hormonal features and asymmetric dimethyl-arginine levels, in women with the polycystic ovary syndrome Atherosclerosis 2007;