Mouse monoclonal antibody to S-Adenosylmethionine Clone 84-3
The Mouse monoclonal antibody to S-Adenosylmethionine Clone 84-3 is For Research Use Only
Storage Instructions: Store at 4°C, -20°C for long term storage
Storage Buffer: PBS 10mM pH 7.4 (NaCl 150mM), Sodium azide 0.02%, BSA 10mg/ml or PBS 10mM pH 7.4 (NaCl 150mM), Sodium azide 0.02%, Glycerol 50%, BSA 10mg/ml
Purity: >95% Purified from mouse ascites fluid by affinity chromatography
Clone Number: 84-3
Immunoglobin Isotype: IgG2b
Affinity: Ka = 7.29 x 1010L/mol (1.37 x 10-11M)
Specificity: Shows the following reactivities with related compounds: S-Adenosylmethionine: 100% ,S-Adenosylhomocysteine: <1% ,Adenosine: <1%, L-Methionine: <1%.
Immunogen: S- Adenosylmethionine analog conjugated to KLH
Applications: The application notes include recommended starting dilutions. Optimal dilutions/concentrations should be determined by the end user. Higher dilution than suggested maybe used in IHC and IF. The product may be used in other not-yet-tested applications.
- cELISA: 1:10000-30000
- FCM: 1:200-400
- IHC: 1:200-400
S- Adenosylmethionine is a common co-substrate involved in methyl group transfers. It is made from adenosine triphosphate (ATP) and methionine by methionine adenosyltransferase. Transmethylation, transsulfuration, and aminopropylation are the metabolic pathways that use SAM. Although these anabolic reactions occur throughout the body, most SAM is produced and consumed in the liver.