Mouse Monoclonal Antibody to S-Adenosylmethionine Clone 118-6
The Mouse Monoclonal Antibody to S-Adenosylmethionine Clone 118-6 is For Research Use Only
Storage Instructions: Store at 4°C, -20°C for long term storage
Storage Buffer: PBS 10mM pH 7.4 (NaCl 150mM), Sodium azide 0.02%, BSA 10mg/ml or PBS 10mM pH 7.4 (NaCl 150mM), Sodium azide 0.02%, Glycerol 50%, BSA 10mg/ml
Purity: >95% Purified from mouse ascites fluid by affinity chromatography
Clone Number: 118-6
Immunoglobin Isotype: IgG2b
Affinity: Ka = 5.75 x 109 L/mol (1.74 x 10-10M)
Specificity: Dosage-dependent competition was detected as a sample was added to a cELISA ( Any SAM from a sample competes with the coated SAM heptan to bind HRP-conjugated antibody 118-6 ) . The sample is the product of the following biochemical reaction: Methionine Adenosyltransferase ( MAT ) was added to methionine and adenosine triphosphate under an appropriate buffer at 37°C. It indicates that antibody 118-6 specifically binds physiologically produced SAM.
Cross-Reactivity: shows the following reactivity with related compounds: SAdenosylmethionine: 100%, S-Adenosylhomocysteine: < 1%, Adenosine: < 1%, LMethionine: < 1%, Methythioadenosine ( MTA): < 1%, ADP ( adenosine diphosphate) < 1%, ATP ( adenosine triphosphate) < 1% S-Adenosylmethionine analog conjugated to KLH. Immunogen: S- Adenosylmethionine analog conjugated to KLH
S- Adenosylmethionine is a common co-substrate involved in methyl group transfers. It is made from adenosine triphosphate (ATP) and methionine by methionine adenosyltransferase. Transmethylation, transsulfuration, and aminopropylation are the metabolic pathways that use SAM. Although these anabolic reactions occur throughout the body, most SAM is produced and consumed in the liver.