Mouse MBL-A ELISA Kit

$690.00$1,120.00

The Eagle Biosciences Mouse MBL-A ELISA Kit is to be used for the quantitative determination of mouse MBL-A in serum, plasma and cell culture supernatant. The Eagle Biosciences Mouse MBL-A ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

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Mouse MBL-A ELISA Kit

For Research Use Only

Sizes: 1×96 wells and 2×96 wells
Sensitivity: 1.2 ng/ml
Standard Range: 1.2-75 ng/ml
Incubation Time: 3.5 hours
Sample Type: Serum, plasma, and cell culture supernatants
Sample Size: 100 µl

Product manufactured in the U.S.A.

Additional Information

Assay Background


Mannose Binding Lectin (MBL) also called mannose- or mannan-binding protein (MBP) is a member of the collectins and an important element in innate immunity. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. It forms a complex with C1r/C1s like serine proteases designated MASP that proteolytically cleave C4, C2 and C3. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. The MBL-MASP pathway (better known as the lectin pathway) is antibody and C1q-independent. MBL exhibits complement-dependent anti-bacterial activity and acts directly as an opsonic and therefore plays an important role in innate immunity. MBL is synthesized by hepatocytes and has been isolated from the liver and serum of several vertebrate species. Only one form of human MBL has been characterized, while two forms are found in rhesus monkeys, rabbits, rats and mice. The murine forms are known as MBL-A and MBL-C. The MBL-C concentrations in serum are about 6-fold higher compared to that of MBL-A. MBL-A, but not MBL-C was found to be an acute phase protein in casein and LPS-injection models. MBL-C exists in higher oligomeric forms than MBL-A. In plasma of healthy mice MBL-A concentrations range from 4 to 12 μg/ml. In infectious diseases the MBL-A concentration was found to increase approximately two-fold.

Assay Principle


The mouse MBL-A ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allow free choice of batch size for the assay. Samples and standards are captured by a solid bound specific antibody. Biotinylated tracer antibody will bind to captured mouse MBL-A. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of citric acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the mouse MBL-A standards (log). The mouse MBL-A concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Manual

Product Manual