Mouse H-FABP ELISA Kit
The Mouse H-FABP ELISA Kit is For Research Use Only
Sizes: 1×96 wells and 2×96 wells
Sensitivity: 400 pg/ml
Standard Range: 390-25,000 pg/ml
Incubation Time: 3.5 hours
Sample Type: Serum and plasma
Sample Size: 100 µl
Alternative Names: Heart Fatty Acid-Binding Protein
Assay Background
Fatty acid-binding proteins (FABPs) are a class of cytoplasmic proteins that bind long chain fatty acids. FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity. They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism. There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression. Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels. Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas.
The H-FABP protein is derived from the FABP3 gene. The FABP content of mouse heart muscle (H-FABP) is markedly high, 10-20 mol% of cytoplasmic proteins. Following acute myocardial infarction (AMI) the small protein H-FABP is rapidly released into the circulation. Significantly elevated serum/plasma concentrations are found within 3 hours after AMI which generally return to normal values within 12 to 24 hours. These features make H-FABP a useful research tool for the early assessment or exclusion of AMI, and for the monitoring of a recurrent infarction. Constitutive H-FABP released from the heart after AMI is quantitatively recovered in serum/plasma. Thus assessment of H-FABP is also a very effective tool for the estimation of the infarct size. The mouse H-FABP kit can also be used for measurement of brain-type FABP, a marker for brain injury detection.
In serum/plasma of healthy human individuals approximately 1.6 ng/ml of H-FABP is present.
