The Mouse H-FABP ELISA Kit is to be used for the quantitative determination of mouse H-FABP in serum or plasma samples. The Eagle Biosciences Mouse H-FABP ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.


The Mouse H-FABP ELISA Kit is For Research Use Only

Sizes: 1×96 wells and 2×96 wells
Sensitivity: 400 pg/ml
Standard Range: 390-25,000 pg/ml
Incubation Time: 3.5 hours
Sample Type: Serum and plasma
Sample Size: 100 µl
Alternative Names: Heart Fatty Acid-Binding Protein

Assay Background

Fatty acid-binding proteins (FABPs) are a class of cytoplasmic proteins that bind long chain fatty acids. FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity. They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism. There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression. Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels. Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas.
The H-FABP protein is derived from the FABP3 gene. The FABP content of mouse heart muscle (H-FABP) is markedly high, 10-20 mol% of cytoplasmic proteins. Following acute myocardial infarction (AMI) the small protein H-FABP is rapidly released into the circulation. Significantly elevated serum/plasma concentrations are found within 3 hours after AMI which generally return to normal values within 12 to 24 hours. These features make H-FABP a useful research tool for the early assessment or exclusion of AMI, and for the monitoring of a recurrent infarction. Constitutive H-FABP released from the heart after AMI is quantitatively recovered in serum/plasma. Thus assessment of H-FABP is also a very effective tool for the estimation of the infarct size. The mouse H-FABP kit can also be used for measurement of brain-type FABP, a marker for brain injury detection.
In serum/plasma of healthy human individuals approximately 1.6 ng/ml of H-FABP is present.

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Additional Information

Assay Principle

The mouse H-FABP ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours for Mouse H-FABP. The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are captured by a solid bound specific antibody. Biotinylated tracer antibody will bind to captured mouse H-FABP. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of citric acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the mouse/rat H-FABP standards (log). The H-FABP concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.


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