MMAE Antibody Drug Conjugate (ADC) ELISA Assay Kit

$1,850.00

The Eagle Biosciences Human MMAE Antibody Drug Conjugate (ADC) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for use in the quantitative determination of antibody-MMAE-conjugate level in test sample. It is useful for pre-clinical and clinical pharmacology study of MMAE Antibody Drug Conjugate (ADC).  Samples from tissue/cell culture and serum samples from human, rat, mouse, primate, etc. can be used directly with this kit.  Both humanized monoclonal antibody based MMAE-ADC and mouse monoclonal antibody based MMAE-ADC can be measured with this kit.

MMAE Antibody Drug Conjugate (ADC) ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.0435 µg/mL
Dynamic Range: 0.032 – 4.0 µg/mL
Incubation Time: 2.5 hours
Sample Type: Serum, Cell Culture, Tissue
Sample Size: 100 µL

Controls Included

Product Developed and Manufactured in the USA

Additional Information

Assay Principle


The Eagle Biosciences MMAE Antibody Drug Conjugate (ADC) ELISA Assay Kit is designed, developed and produced for the quantitative measurement of antibody Monomethyl auristatin E (MMAE) conjugate in serum. The assay utilizes the competitive immunoassay technique with an antibody that exclusively binds to Monomethyl auristatin E.

Assay calibrators (antibody MMAE conjugate) and test serum samples are added directly to wells of a microtiter plate that is coated with specific anti-MMAE antibody. Subsequently, a horseradish peroxidase (HRP) conjugated MMAE is added to each well. During the incubation period, the antibody MMAE conjugate competes with the HRP conjugated MMAE for the limited binding sites of anti-MMAE antibody. An immune complex of well coated “anti-MMAE antibody – HRP conjugated MMAE” is formed. The unbound antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction, which is terminated with an acidic reagent (i.e. ELISA stop solution). The absorbance is then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is inversely proportional to the amount of antibody-MMAE conjugate in the test sample. A calibration curve is generated by plotting the absorbance versus the respective antibody-MMAE conjugate concentration for each calibrator on a 4-parameter or log-logit curve fitting. The concentration of antibody-MMAE conjugate in test samples is determined directly from this calibration curve.

  1. Place a sufficient number of Anti-MMAE antibody coated microwell strips/wells in a holder to run human Anti-MMAE standards, controls and unknown samples in duplicate.
  2. Add 100 µL of calibrators and test samples into the designated microwells.
  3. Seal the plate wells securely, cover with foil or other material to protect from light, and rotate on an ELISA plate shaker (small orbit radius) for 30 minutes at 400 to 450 rpm.
  4. Immediately add 25 µL of HRP Conjugated MMAE (cat# 30719) to each well. (Note: no wash step before add the HRP conjugated MMAE)
  5. Seal the plate wells securely, cover with foil or other material to protect from light, and rotate on an ELISA plate shaker (small orbit radius) for 2 hr. ± 10 minutes at 400 to 450 rpm.
  6. Wash each well 5 times by dispensing 350 µL of working wash solution into each well and then completely aspirating the contents.
  7. Alternatively, an automated microplate washer can be used.
  8. Add 100 µL of ELISA HRP Substrate into each of the wells.
  9. Cover the plate with aluminum foil or other material to avoid exposure to light. Incubate plate static, at room temperature for 20 minutes.
  10. Immediately add 100 µL of ELISA Stop Solution into each of the wells. Mix gently.
  11. Read the absorbance at 450 nm.

Typical Standard Curve


MMAE Antibody Drug Conjugate (ADC) ELISA Assay Kit

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