MDA Oxidized LDL ELISA
MDA Oxidized LDL ELISA Developed and Manufactured in Austria by Biomedica
Size: 1×96 wells
Sensitivity: 0.05 µg/ml
Dynamic Range: 0 – 10 µg/ml
Incubation Time: 3.5 hours
Sample Type: Serum, Plasma
Sample Size: 20 µL
Alternative Names: Human MDA Oxidized Low Density Lipoprotein, MDA Oxidized Low Density Lipoprotein, Human Malondialdehyde Oxidized LDL
For Research Use Only
Reference Range: Serum samples of 71 blood donors had a median level 1.0 µg/ml. Each laboratory should establish its own reference range for the samples under investigation.
The MDA-oxLDL ELISA kit is a sandwich enzyme immunoassay for the quantitative determination of human MDA-oxLDL adducts in serum and plasma (EDTA, heparın, citrate) samples. In a first step, assay buffer and standard/control/sample are pipetted into the wells of the microtiter-strips, which are pre-coated with monoclonal mouse anti-human MDA-oxLDL antibody. MDA-oxLDL present in the standard/control/sample is captured by the pre-coated antibody in the well. In the washing step all non-specific unbound material is removed. In a second step, the conjugate (monoclonal mouse anti-human MDA-oxLDL-HRP) is pipetted into the wells and forms a sandwich with the MDA-oxLDL bound by the capture antibody on the plate. After another washing step, the substrate (TMB, tetramethylbenzidine) is pipetted into the wells. The enzyme-catalyzed color change of the substrate is directly proportional to the amount of MDA-oxLDL. This color change is detectable with a standard microtiter plate reader. A dose response curve of the absorbance (optical density, OD at 450 nm) vs. standard concentration is generated, using the values obtained from the standard. The concentration of MDA-oxLDL in the sample is determined directly from the dose response curve.