MDA Oxidized LDL ELISA

$595.00

The MDA Oxidized LDL ELISA Assay kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human MDA Oxidized LDL in serum or plasma. The Eagle Biosciences MDA Oxidized LDL ELISA Assay kit is for research use only and not to be used in diagnostic procedures.

SKU: BI-20022 Categories: , ,

MDA Oxidized LDL ELISA

MDA Oxidized LDL ELISA Developed and Manufactured in Austria by Biomedica

Size: 1×96 wells
Sensitivity: 0.05 µg/ml
Dynamic Range: 0 – 10 µg/ml
Incubation Time: 3.5 hours
Sample Type: Serum, Plasma
Sample Size: 20 µL
Alternative Names: Human MDA Oxidized Low Density Lipoprotein, MDA Oxidized Low Density Lipoprotein, Human Malondialdehyde Oxidized LDL
For Research Use Only

Controls Included

Reference Range: Serum samples of 71 blood donors had a median level 1.0 µg/ml. Each laboratory should establish its own reference range for the samples under investigation.

Assay Principle

The MDA-oxLDL ELISA kit is a sandwich enzyme immunoassay for the quantitative determination of human MDA-oxLDL adducts in serum and plasma (EDTA, heparın, citrate) samples. In a first step, assay buffer and standard/control/sample are pipetted into the wells of the microtiter-strips, which are pre-coated with monoclonal mouse anti-human MDA-oxLDL antibody. MDA-oxLDL present in the standard/control/sample is captured by the pre-coated antibody in the well. In the washing step all non-specific unbound material is removed. In a second step, the conjugate (monoclonal mouse anti-human MDA-oxLDL-HRP) is pipetted into the wells and forms a sandwich with the MDA-oxLDL bound by the capture antibody on the plate. After another washing step, the substrate (TMB, tetramethylbenzidine) is pipetted into the wells. The enzyme-catalyzed color change of the substrate is directly proportional to the amount of MDA-oxLDL. This color change is detectable with a standard microtiter plate reader. A dose response curve of the absorbance (optical density, OD at 450 nm) vs. standard concentration is generated, using the values obtained from the standard. The concentration of MDA-oxLDL in the sample is determined directly from the dose response curve.

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Related News:

MDA Oxidized LDL ELISA Assay Published in Obesity Research and Clinical Practice Journal

Additional Information

Assay Procedure

  1. Add 100 µl ASYBUF (Assay buffer) into each well.
  2. Add 20 µl STD/CTRL/SAMPLE (Standard/Control/Sample) in duplicate into respective wells, except blank.
  3. Cover the strips tightly and incubate for 90 min at room temperature (18-26°C).
  4. Aspirate and wash wells 5x with 300 µl diluted WASHBUF (Wash buffer). Remove remaining WASHBUF by hitting plate against paper towel after the last wash.
  5. Add 100 µl CONJ (Conjugate) into each well, except blank.
  6. Cover the strips tightly and incubate for 90 min at room temperature (18-26°C).
  7. Aspirate and wash wells 5x with 300 µl diluted WASHBUF (Wash buffer). Remove remaining WASHBUF by hitting plate against paper towel after the last wash.
  8. Add 100 µl SUB (Substrate) into each well.
  9. Incubate for 30 min at room temperature (18-26°C) in the dark.
  10. Add 50 µl STOP (Stop solution) into each well, shake well.

Typical Standard Curve

MDA Oxidized LDL ELISA Standard Curve

Documents

Product Documents

Publications

Citations

Laguna-Camacho, Antonino. “Direct effects of fatty meals and adiposity on oxidised low-density lipoprotein.” Obesity Research & Clinical Practice 2015: 9(3): 298-300.

References

  • Oxidised low-density lipoprotein concentration – early marker of an altered lipid metabolism in young women with PCOS. Macut D et al, Eur J Endocrinol. 2006; 155(1):131-136
  • Colocalisation of intraplaque C reactive protein, complement, oxidised low density lipoprotein, and macrophages in stable and unstable angina and acute myocardial infarction. Meuwissen M et al, J Clin Pathol. 2006; 59(2):196-201
  • LDL size, total antioxidant status and oxidised LDL in normal human pregnancy: a longitudinal study. Belo L et al, Atherosclerosis. 2004; 177(2):391-399
  • Workshop/Conference Report – Quantitative Bioanalytical Methods Validation and Implementation: Best Practices for Chromatographic and Ligand Bindig Assays. Viswanathan CT et al, The AAPSJournal 2007; 9(1) Article 4

Product Citations