MANF ELISA Assay Kit
The MANF ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.25 pg/ml
Dynamic Range: 30 – 75 pg/mL
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma, Cell Culture Supernatant
Sample Size: 100 µL
Alternative Name: Mesencephalic astrocyte-derived neurotrophic factor, hMANF
The kit should be stored at +2…+6°C. Unopened, the kit will remain stable until the expiry date printed on the kit label. The expiry date of each unopened component is printed on the label of the individual component. After opening, the components should be used within 8 weeks (microwell plate desiccation recommended).
The human MANF Assay kit is based on the quantitative sandwich enzyme immunoassay technique. Microtiter wells are pre-coated with human MANF-specific monoclonal capture antibodies. Samples and standards are pipetted into microwells and human MANF molecules present in the sample are bound by the capture antibodies. After incubation, unbound material is removed by washing the wells. Then, horseradish peroxidase (HRP) conjugated human MANF-specific polyclonal detection antibodies bind to different epitopes of human MANF molecules. After washing, the ready to use HRP substrate (TMB) is added to wells. The intensity of the colour produced is directly proportional to the amount of human MANF in the sample. Colour development is then stopped by the addition of stop solution. Absorbance is measured at 450 nm.