Immunology ELISA Assay Kit

Latex Hev b5 ELISA Assay

$1,020.00

The Latex Hev b5 ELISA Assay test is the first commercial quantitative test to measure Hev b 5 immunologically in NRL products. By the use of specific monoclonal antibodies, sensitivity and specificity stay high even in the presence of other proteins or chemical substances derived from the manufacturing process of Natural Rubber Products (NRL) products.

SKU: LAb531-K01 Categories: , ,

Latex Hev b5 ELISA Assay

The Latex Hev b5 ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.5 µg/L
Dynamic Range: 10 -1000 µg/L
Incubation Time: 2 hours, 45 minutes
Sample Type: Rubber products
Sample Size: 5 µL
Alternative Name: FITkit Hev b 5 Latex Allergen ELISA

Storage Conditions

The kit should be stored at +2…+8 °C.
The unopened kit is stable until the expiry date, printed on the kit label. The expiry date of each unopened component is printed on the label of the individual component.
Once opened, the microwell plate and liquid components are stable for eight weeks at +2…+8 °C.
After reconstitution, the control should be used during the same working day.
After dilution of the PBS Wash Concentrate, the washing solution is stable for eight weeks at room temperature (RT).

Assay Principle

FITkit® Hev b 5 test is based on the immunoenzymetric assay technique. Microtiter wells are coated with Hev b 5-specific monoclonal capture antibody that during the first incubation binds Hev b 5 molecules from the sample extract. After incubation, unbound material is removed by washing the wells. During the second incubation, horseradish peroxidase (HRP) labelled Hev b 5-specific monoclonal detection antibody binds to a different epitope of Hev b 5 molecules. As such, the two antibodies are a matched pair that forms a “sandwich” around the target Hev b 5 allergen. After washing, HRP substrate is added and the intensity of the colour produced is directly proportional to the Hev b 5 concentration of the sample.

Related Products

Latex Allergen 4-in-1 ELISA
Latex Hev b3 ELISA Assay
Latex Hev b 6.02 ELISA Assay

Additional Information

Assay Background

The Eagle Biosciences FITkit® is an ELISA test for measuring natural rubber latex (NRL) allergens from a variety of rubber products, such as gloves, condoms, teats, etc. FITkit® technology has been developed by the leading scientists working in the field of NRL allergy, in cooperation with major glove manufacturers. It shows a good correlation to the allergen content of gloves, measured by currently availabe patient IgE methods.

This novel method is the first ever test for the measurement of clinically relevant NRL allergens, and enables quantification of individual allergens. It overcomes the significant limitations of other tests by using highly purified and characterized allergens, and specific monoclonal antibodies, against four major latex allergens (Hev b 1, Hev b 3, Hev b 5 and Hev b 6.02) known to be present in NRL products.

Assay Procedure

  1. Dispense 100 μL of Assay Buffer into each well.
  2. Pipette 25 μL of calibrator, control and sample into appropriate wells in duplicate.
  3. Cover the microwell plate. Incubate the microwell plate for 60 minutes at room temperature on a microwell plate shaker (100-200 rpm).
  4. Aspirate and wash the wells 4 times with 300 μL of washing solution.
  5. Dispense 100 μL of Enzyme Conjugate into the wells in duplicate.
  6. Cover the microwell plate. Incubate the microwell plate for 30 minutes at room temperature on a microwell plate shaker (100-200 rpm).
  7. Aspirate and wash the wells 4 times with 300 μL of washing solution.
  8. Add 100 μL of HRP Substrate Solution at fixed time points into each well.
  9. Cover the microwell plate. Incubate the microwell plate for 15 minutes at room temperature on a microwell plate shaker (100-200 rpm).
  10. Stop the reaction by adding 100 μL of Stopping Solution into each well at the same fixed time points as in Step 8 so that exactly the same substrate reaction time is achieved. Shake the microwell plate for 1-2 minutes to mix the solutions.
  11. Measure the absorbance at 414 nm using a microwell plate or strip reader, preferably immediately but no more than 60 minutes after stopping the reaction. If the microwell plate is not read immediately, protect the microwell plate from light.

Typical Standard Curve

Latex Hev b5 ELISA Assay

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