Latex Hev b3 ELISA Assay
The Latex Hev b3 ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 2.3 µg/L
Dynamic Range: 10 -1000 µg/L
Incubation Time: 2 hours
Sample Type: Rubber products
Sample Size: 5 µL
Alternative Name: FITkit Hev b3 Latex Allergen ELISA
The kit should be stored at +2…+8 °C.
The unopened kit is stable until the expiry date, printed on the kit label. The expiry date of each unopened component is printed on the label of the individual component.
Once opened, the microwell plate and liquid components are stable for eight weeks at +2…+8 °C.
After reconstitution, the control should be used during the same working day.
After dilution of the PBS Wash Concentrate, the washing solution is stable for eight weeks at room temperature (RT).
FITkit® Hev b 3 test is based on the immunoenzymetric assay technique. Microtiter wells are coated with Hev b 3-specific monoclonal capture antibody that during the first incubation binds Hev b 3 molecules from the sample extract. After incubation, unbound material is removed by washing the wells. During the second incubation, horseradish peroxidase (HRP)
labelled Hev b 3-specific monoclonal detection antibody binds to a different epitope of Hev b 3 molecules. As such, the two antibodies are a matched pair that forms a “sandwich” around the target Hev b 3 allergen. After washing, HRP substrate is added and the intensity of the colour produced is directly proportional to the Hev b 3 concentration of the sample.