Latex Hev b 6.02 ELISA Assay

$1,020.00

The Eagle Biosciences FITkit® Hev b 6.02 test is the first commercial quantitative test to measure Hev b 6.02 immunologically in NRL products. By the use of specific monoclonal antibodies, sensitivity and specificity stay high even in the presence of other proteins or chemical substances derived from the manufacturing process of Natural Rubber Products (NRL) products.

SKU: LAb631-K01 Categories: , ,

Latex Hev b 6.02 ELISA Assay

The Latex Hev b 6.02 ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.1 µg/L
Dynamic Range:10 -1000 µg/L
Incubation Time: 2 hours
Sample Type: Rubber products
Sample Size: 5 µL
Alternative Names: Latex Allergen – FITkit Hev b 6.02


Assay Background

The FITkit® is an ELISA test for measuring natural rubber latex (NRL) allergens from a variety of rubber products, such as gloves, condoms, teats, etc. FITkit® technology has been developed by the leading scientists working in the field of NRL allergy, in cooperation with major glove manufacturers. It shows a good correlation to the allergen content of gloves, measured by currently availabe patient IgE methods.
This novel method is the first ever test for the measurement of clinically relevant NRL allergens, and enables quantification of individual allergens. It overcomes the significant limitations of other tests by using highly purified and characterized allergens, and specific monoclonal antibodies, against four major latex allergens (Hev b 1, Hev b 3, Hev b 5 and Hev b 6.02) known to be present in NRL products.


Related products

Latex Allergen ELISA Assay Kit – FITkit Hev b 1
Latex Allergen ELISA Assay Kit – FITkit Hev b 3
Latex Allergen 4-in-1 ELISA

Additional Information

Assay Principle


FITkit Hev b 6.02 test is based on the immunoenzymetric assay technique. Microtiter wells are coated with Hev b 6.02-specific monoclonal capture antibody that during the first incubation binds Hev b 6.02 molecules from the sample extract. After incubation, unbound material is removed by washing the wells. During the second incubation, horseradish peroxidase (HRP) labelled Hev b 6.02-specific monoclonal detection antibody binds to a different epitope of Hev b 6.02 molecules. As such, the two antibodies are a matched pair that forms a “sandwich” around the target Hev b 6.02 allergen. After washing, HRP substrate is added and the intensity of the colour produced is directly proportional to the Hev b 6.02 concentration of the sample.

Assay Procedure


  1. Dispense 100 μL of Assay Buffer into each well.
  2. Pipette 25 μL of calibrator, control and sample into appropriate wells in duplicate.
  3. Cover the microwell plate. Incubate the microwell plate for 60 minutes at room temperature on a microwell plate shaker (100-200 rpm).
  4. Aspirate and wash the wells 4 times with 300 μL of washing solution.
  5. Dispense 100 μL of Enzyme Conjugate into the wells in duplicate.
  6. Cover the microwell plate. Incubate the microwell plate for 30 minutes at room temperature on a microwell plate shaker (100-200 rpm).
  7. Aspirate and wash the wells 4 times with 300 μL of washing solution.
  8. Add 100 μL of HRP Substrate Solution at fixed time points into each well.
  9. Cover the microwell plate. Incubate the microwell plate for 15 minutes at room temperature on a microwell plate shaker (100-200 rpm).
  10. Stop the reaction by adding 100 μL of Stopping Solution into each well at the same fixed time points as in Step 8 so that exactly the same substrate reaction time is achieved. Shake the microwell plate for 1-2 minutes to mix the solutions.
  11. Measure the absorbance at 414 nm using a microwell plate or strip reader, preferably immediately but no more than 60 minutes after stopping the reaction. If the microwell plate is not read immediately, protect the microwell plate from light.

Typical Standard Curve


Latex Hev b 6.02 ELISA Assay

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