Latex Allergen 4-in-1 ELISA
The Latex Allergen 4-in-1 ELISA is For Research Use Only
Size: 4×96 wells
Dynamic Range: Hev b1, Hev b3: 10-1000 μg/L; Hev b 5: 5 – 100 μg/L; Hev b 6.02: 5 – 200 μg/L
Incubation Time: 2 hours
Sample Type: Rubber products
Sample Size: 25 µL
The Latex Allergen 4-in-1 Assay Kit is based on the enzyme immunometric assay technique. Microtiter wells are pre-coated with one of 4 Hev b specific monoclonal antibody. Calibrators, controls and samples are pipetted into the wells and Hev b allergen is bound by immobilized antibody. After incubation, unbound material is removed by washing the wells. In the second incubation, horseradish peroxidase (HRP) labeled Hev b specific monoclonal antibodies are added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Hev b allergen bound in the initial step. The color development is stopped and the intensity of the color is measured.