IL-6 ELISA Assay Kit

$560.00

The IL-6 ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IL-6 in human biological samples. The Eagle Biosciences Human Interleukin 6 (IL-6) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: IL631-K01 Categories: , ,

IL-6 ELISA Assay Kit

The IL-6 ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 6.25 pg/mL
Dynamic Range: 6.25-400 pg/ml
Incubation Time: 2 hours
Sample Type: Biological Samples
Sample Size: 100 µl
Alternative Names: Interleukin 6, IL6


Assay Background

Interleukin 6 (IL-6) is a multifunctional protein produced by lymphoid and non-lymphoid cells and by normal and transformed cells, including T cells, monocyte/macrophages, fibroblasts, hepatocytes, vascular endothelial cells, cardiac myxomas, bladder cell carcinomas, myelomas, astrogliomas and glioblastomas. The production of IL-6 in these cells is regulated, either positively or negatively, by a variety of signals including mitogens, antigenic stimulation, lipopolysaccharides, IL-1, TNF, PDGF and viruses. For reviews on IL-6, see references 1-5.

The human IL-6 cDNA sequence predicts a protein of 212 amino acid (aa) residues in length with two potential N-glycosylation sites. The hydrophobic N-terminal 28 aa residue signal peptide is cleaved to produce a mature protein of 184 amino acids with four cysteine residues and a predicted molecular mass of 21 kDa. The mouse IL-6 cDNA sequence shows a homology of 42% at the aa level when compared with the human sequence. On the basis of sequence similarity and gene structural motif similarity, IL-6 can be grouped in a family of cytokines that also includes OSM, G-CSF, LIF, and CNTF. All of these cytokines are predicted to have a four helix bundle structure similar to that found for growth hormone, suggesting that they all evolved from a common ancestral gene.

The effects of IL-6 on different cells are numerous and varied. The effect on B cells is stimulation of differentiation and antibody secretion. IL-6 also affects T cells, acting as a co-stimulant with sub-optimal concentrations of PHA or Con A to stimulate IL-2 production and IL-2 receptor expression.  IL-6 exhibits growth factor activity for mature thymic or peripheral T-cells and reportedly enhances the differentiation of cytotoxic T-cells in the presence of IL-2 or IFN-γ. IL-6 stimulates production of acute phase proteins by hepatocytes and has colony-stimulating activity on hematopoietic stem cells. IL-6 has growth factor activities and will stimulate the growth of myeloma/hybridoma/ plasmacytoma cells, EBV-transformed B cells, keratinocytes and mesangial cells.


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Additional Information

Assay Principle


In this assay the interleukin-6 (IL-6) present in samples reacts with the anti-IL-6 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-IL-6, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IL-6 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IL-6 in the test sample. The quantity of IL-6 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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Citations


Amer, W., Morsi, A., Alnoury, H. Cervical Cerclage for Pregnant Women at High-risk of Recurrent Preterm Birth can Act Beyond its Mechanical Action. Women’s Health Journal. (2021)11; 2:196-202.

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