IL-6 ELISA Assay Kit


The IL-6 ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IL-6 in human biological samples. The Eagle Biosciences Human Interleukin 6 (IL-6) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: IL631-K01 Categories: , ,

IL-6 ELISA Assay Kit

The IL-6 ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 6.25 pg/mL
Dynamic Range: 6.25-400 pg/ml
Incubation Time: 2 hours
Sample Type: Biological Samples
Sample Size: 100 µl
Alternative Names: Interleukin 6, IL6

Assay Background

Interleukin 6 (IL-6) is a multifunctional protein produced by lymphoid and non-lymphoid cells and by normal and transformed cells, including T cells, monocyte/macrophages, fibroblasts, hepatocytes, vascular endothelial cells, cardiac myxomas, bladder cell carcinomas, myelomas, astrogliomas and glioblastomas. The production of IL-6 in these cells is regulated, either positively or negatively, by a variety of signals including mitogens, antigenic stimulation, lipopolysaccharides, IL-1, TNF, PDGF and viruses. For reviews on IL-6, see references 1-5.

The human IL-6 cDNA sequence predicts a protein of 212 amino acid (aa) residues in length with two potential N-glycosylation sites. The hydrophobic N-terminal 28 aa residue signal peptide is cleaved to produce a mature protein of 184 amino acids with four cysteine residues and a predicted molecular mass of 21 kDa. The mouse IL-6 cDNA sequence shows a homology of 42% at the aa level when compared with the human sequence. On the basis of sequence similarity and gene structural motif similarity, IL-6 can be grouped in a family of cytokines that also includes OSM, G-CSF, LIF, and CNTF. All of these cytokines are predicted to have a four helix bundle structure similar to that found for growth hormone, suggesting that they all evolved from a common ancestral gene.

The effects of IL-6 on different cells are numerous and varied. The effect on B cells is stimulation of differentiation and antibody secretion. IL-6 also affects T cells, acting as a co-stimulant with sub-optimal concentrations of PHA or Con A to stimulate IL-2 production and IL-2 receptor expression.  IL-6 exhibits growth factor activity for mature thymic or peripheral T-cells and reportedly enhances the differentiation of cytotoxic T-cells in the presence of IL-2 or IFN-γ. IL-6 stimulates production of acute phase proteins by hepatocytes and has colony-stimulating activity on hematopoietic stem cells. IL-6 has growth factor activities and will stimulate the growth of myeloma/hybridoma/ plasmacytoma cells, EBV-transformed B cells, keratinocytes and mesangial cells.

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Additional Information

Assay Principle

In this assay the interleukin-6 (IL-6) present in samples reacts with the anti-IL-6 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-IL-6, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IL-6 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IL-6 in the test sample. The quantity of IL-6 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


Product Documents



Amer, W., Morsi, A., Alnoury, H. Cervical Cerclage for Pregnant Women at High-risk of Recurrent Preterm Birth can Act Beyond its Mechanical Action. Women’s Health Journal. (2021)11; 2:196-202.

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