IgG Allergy (22 Foods) ELISA Assay Kit

$155.00

The Eagle Biosciences IgG Allergy (22 foods) ELISA Assay Kit is intended for use in the semi-quantitative determination of IgG-mediated food sensitivities of 20 food antigens in human serum and plasma. The Eagle Biosciences IgG Allergy (22 foods) ELISA Assay Kit is intended for research use only.

SKU: CNS129 Categories: , ,

IgG Allergy (22 Foods) ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: Cut-off
Dynamic Range: Negative <8 AU/ml, Borderline 8-12.5 AU/ml, Positive 12.5+
Incubation Time: 1.25 hours
Sample Type: Serum, Plasma
Sample Size: 10 µL

Controls Included

Product Developed and Manufactured in the UK by Omega Diagnostics

Additional Information

Assay Background

Many people exhibit chronic food sensitivity reactions to specific food antigens. Unlike the immediate effects of IgE-mediated allergy, IgG-mediated food sensitivity reactions may take several days to appear. Controlled removal of the problem foods from the patient’s diet will, in many cases, rapidly improve the patient’s condition. General lethargy, weight gain, dermatitis, arthritis and tiredness are associated with food allergies. Irritable bowel syndrome may also be linked to food sensitivity.

Assay Principle

Diluted serum samples are incubated with antigen extracts from 22 different foods immobilised on microtitre wells. After washing away unbound serum components, goat anti-human IgG conjugated to horseradish peroxidase is added to the wells,and this binds to surface-bound antibodies in the second incubation. Unbound conjugate is removed by washing, and a solution containing 3,3’,5,5’-tetramethylbenzidine (TMB) and enzyme substrate is added to trace specific antibody binding. Addition of Stop Solution terminates the reaction and provides the appropriate pH for colour development. The optical densities of the standards, positive control and samples are measured using a microplate reader at 450nm.

Assay Procedure

  1. Add 10µl of Sample to one vial of Diluent (Reagent 1)
  2. Dispense Standards, the Positive Control and the diluted sample intothe specified microplate wells
  3. Incubate for 30 minutes at room temperature.
  4. Wash the wells three times
  5. Dispense 100µl of Conjugate (Reagent 3) into each well
  6. Incubate at room temperature for 30 minutes
  7. Wash the wells four times
  8. Add 100µl of TMB Substrate (Reagent 4) to each well
  9. Incubate at room temperature for 10 minutes
  10. Add 100µl Stop Solution (Reagent 5) to each well
  11. Read the optical density at 450nm (single wavelength) or 450/620nm (dual wavelength).

List of Allergens


Manual

Product Manual