Human MAp44 ELISA Kit


The Human MAp44 ELISA Kit is to be used for the quantitative determination of human MAp44 in serum and plasma samples. The Eagle Biosciences Human MAp44 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

Human MAp44 ELISA Kit

The Human MAp44 ELISA Kit is For Research Use Only

Sizes: 1×96 wells and 2×96 wells
Sensitivity: 1.6 ng/ml
Standard Range: 1.6 to 100 ng/ml
Incubation Time: 3.5 hours
Sample Type: Serum and plasma
Sample Size: 100 µl
Alternative Names: MAp1, MBL-associated protein 44

Assay Background

The complement system is an essential part of the innate immune response. It consists of more than 30 proteins which act as a proteolytic cascade as first defense against pathogens. Complement modulates also B- and T-cell responses thereby bridging innate and adaptive immunity. Complement is activated in three ways, the classical, alternative and lectin pathways. The lectin pathway is initiated by pathogen-recognition receptors mannan-binding lectin (MBL), H-ficolin, L-ficolin, M-ficolin or collectin-K1 which recognize a broad range of pathogens or apoptotic cells. All molecules form complexes with MBL-associated serine proteases (MASPs). Five MASP proteins have thus far been identified: MASP1, MASP2, MASP3 and their truncated proteins MAp44 (also called MAp1) and MAp19 (or sMAP). MASP1&3 and MAp44 are all derived from MASP1 gene. MAp44 mRNA consists of the first 8 exons encoding the first four domains identical to those of MASP-1 and MASP-3 and an extra exon encoding 17 amino acids unique to MAp44. Deficiencies in the lectin pathway are associated with sensitivity for infection and the lectin-MASP complex is also involved in activation of the coagulation system. The function of MASP3, MAp44 and MAp19 in the lectin pathway are largely unknown. Competition of recombinant MAp44 in complex with either recombinant MBL or recombinant H-ficolin causes decreased levels of cleavage of C4 in sera depleted of MBL and H-ficolin, respectively, suggesting that MAp44 has an inhibition effect on activation of complement system. MAp44 may represent a tissue-specific protective mechanism by which local inflammation is prevented. MBL-associated protein MAp44 has shown cardio-protective effects in murine models. Although associated with cardiovascular risk factors, MAp44 levels were not associated with severity of disease in humans. In human plasma concentrations are 0.8-3.2 μg/ml. The assay captures MAp44 with a monoclonal antibody recognizing the C-terminus and uses a detection antibody reacting with the CCP-1 domain.

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Additional Information

Assay Principle

The human MAp44 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human MAp44. Biotinylated tracer antibody will bind to the captured human MAp44. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human MAp44 standards (log). The human MAp44 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.


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