Human MANF Rabbit Polyclonal Antibody

$610.00

Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant.

SKU: 310-100 Categories: ,

Human MANF Rabbit Polyclonal Antibody

The Human MANF Rabbit Polyclonal Antibody is For Research Use Only

Immunogen: Human MANF
Uniprot ID: P55145
Alternative Names: ARMET, ARP
Clonality: Rabbit polyclonal
Class: IgG
Reactivity: Human MANF
Application: ELISA, WB
Purification: MANF-affinity purified
Buffer: Concentrated ammonium sulphate in PBS pH 7.4.
Concentration: 0.45 mg/ml
Unit Size: 100 µg
Related Products: Monoclonal and polyclonal antibodies to human MANF.
Shipping: This product is shipped in non-frozen liquid form in ambient conditions.
Storage: Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use
Alternative Names: Mesencephalic astrocyte-derived neurotrophic factor


Protocol: ELISA 1:200 000; Western immunoblotting 1:2000 – 1:6000, IHC, IF
No cross-reactivity in MANF-knockout mouse. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB.   Polyclonal antibody working titer has to be established practically for each particular antigen and assay format.

Background: MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta


Related Products

Human MANF Mouse Monoclonal Antibody Clone 1G12 Isoptype IgG
Human Recombinant MANF Protein
Product Developed and Manufactured by Icosagen

Additional Information

Figure 1:
Human MANF Rabbit Polyclonal Antibody

Western Blot testing of anti-MANF polyclonal antibody. Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Purified recombinant MANF (1 µg per line) expressed using CHOEBNALT85  cell line.