Human Lactoferrin ELISA Assay

$510.00

The Human Lactoferrin ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Lactoferrin in human biological samples.

SKU: SLF31-K01 Categories: , ,

Human Lactoferrin ELISA Assay

The Human Lactoferrin ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.725 ng/ml
Dynamic Range: 3.125 ng/ml – 100 ng/ml
Incubation Time: 70 minutes
Sample Type: Plasma, Serum
Sample Size: 100 μL


Assay Principle

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Lactoferrin present in samples reacts with the anti-Lactoferrin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-LF antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound LF. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of LF in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of LF in the test sample. The quantity of LF in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


SPECIMEN COLLECTION AND HANDLING
Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze thaw cycles.


Related Products

Human Transferrin ELISA Assay Kit
Lactoferrin ELISA Assay Kit

Additional Information

Assay Background


Lactoferrin (LF) is a multifunctional iron-binding protein with antimicrobial activities. It is found in milk and other mucosal secretions. Lactoferrin is known to be part of the innate defense against infection and inflammation and also has function in regulation of iron absorption.

Assay Principle


The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Lactoferrin present in samples reacts with the anti-Lactoferrin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-LF antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound LF. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of LF in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of LF in the test sample. The quantity of LF in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Typical Standard Curve


Human Lactoferrin ELISA Assay

Documents

Product Documents


Product Citations