Human IL-18 ELISA Assay Kit
The Human IL-18 ELISA Assay Kit is For Research Use Only
Size: 1 x 96 wells
Sensitivity: 0.46 pg/mL (50 uL of Sample) 2.13 pg/mL (10 uL of Sample)
Standard Range: 6.86 – 5000 pg/mL
Incubation Time: 2 hours
Sample Type: Serum, Plasma, Urine, and other sample types
Sample Size: 50 uL or 10 uL
Alternative Names: Interleukin 18
Standard Curve Preparation:
Reconstitute Human IL-18 Standard by addition of distilled water as S1. Reconstitution volume is stated on the label of the standard vial. Swirls or mix gently to ensure complete and homogenous solubilization (concentration of reconstituted standard = 5,000 pg/mL). Allow the standard to reconstitute for 10-30 minutes. Mix well prior to making dilutions.
Pipette 200 µL of Sample Diluent into each tube. Use the high standard to product a 1:2 dilution series. Mix each tube thoroughly before the next transfer. Sample Diluent serves as the zero standard (0 pg/mL).
Note: After the kit is opened, the stabilization period of each content is 30 days, so please use the kit within 30 days after opening.
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