Human IgG ELISA Assay

$415.00

The Eagle Biosciences Human IgG ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgG in Human Biological Samples. The Human IgG ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

Human IgG ELISA Assay

The Human IgG ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 1.816 ng/ml
Dynamic Range: 15.6 ng/ml – 500 ng/ml
Incubation Time: 90 minutes
Sample Type: Milk, Plasma, Serum, Tissue Culture Fluids, Urine
Sample Size: 100 μL


Assay Principle

In this Human IgG ELISA assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


Specimen Collection and Handling
Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze thaw cycles.


Related Products

Human IgM ELISA Assay Kit
Human IgE ELISA
Human IgD ELISA Assay

Additional Information

Typical Standard Curve


Human IgG ELISA Assay

Package Inserts


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.