Human Ficolin-1 ELISA Kit

$750.00$1,190.00

The Eagle Biosciences Human Ficolin-1 ELISA Kit is to be used for the quantitative determination of human Ficolin-1 in serum and plasma samples. The Eagle Biosciences Human Ficolin-1 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

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Human Ficolin-1 ELISA Kit

For Research Use Only

Sizes: 1×96 wells and 2×96 wells
Sensitivity: 3.1 ng/ml
Standard Range: 3.1 to 200 ng/ml
Incubation Time: 3.5 hours
Sample Type: Serum and plasma
Sample Size: 100 µl

Product manufactured in the U.S.A.

Additional Information

Assay Background


Ficolins are a family of pattern recognition molecules containing both a collagen-like domain and a fibrinogen-like domain that are capable of activating the complement system, through association with MASPs, on the surface of microorganisms. In human, three Ficolins are known: Ficolin-1 (M-ficolin), Ficolin-2 (L-ficolin), and Ficolin-3 (H-ficolin). Ficolin-1, is mainly expressed by monocytes, granulocytes and lung cells and is present locally in areas of inflammation. However, it was recently also found in serum suggesting that Ficolin-1 also has a role in systemic immunity like Ficolin-2 and Ficolin-3. Functional analysis of Ficolin-1 has demonstrated that it binds specifically to capsular polysaccharides of some microorganisms. Furthermore, it was shown that Ficolin-1 facilitates the uptake of E. coli by a leukemic monocyte lymphoma cell line, suggesting that it functions as a phagocytic receptor on the surface of circulating monocytes. Low cord blood concentration of Ficolin-1 has been associated with higher necrotizing enterocolitis-associated fatality in premature infants and polymorphisms in the Ficolin-1 gene are associated with the development of rheumatoid arthritis and susceptibility to leprosy.

Assay Principle


The human Ficolin-1 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human Ficolin-1. Biotinylated tracer antibody will bind to the captured human M-ficolin. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human Ficolin-1 standards (log). The human Ficolin-1 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Manual

Product Manual