Human CD117 ELISA Assay Kit

$490.00

The Eagle Biosciences Human CD117 ELISA is to be used for the in-vitro quantitative determination of Stem Cell Factor receptor molecule (CD117) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant CD117. The Eagle Biosciences Human CD117 ELISA Assay kit is for Research Use Only.

SKU: C1731-K01 Categories: , ,

Human CD117 ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.095 ng/ml
Dynamic Range: 0.312 – 10 ng/ml
Incubation Time: 1h 45min
Sample Type: Serum, Plasma, Cell Culture
Sample Size: 100 µl

Product manufactured in France by Diaclone

Additional Information

Assay Principle

The CD117 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD117 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD117 concentrations and unknowns are pipetted into these wells. During the first incubation, the CD117 antigen and a biotinylated monoclonal antibody specific for CD117 are simultaneously incubated.  After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD117 present in the samples.

  1. Add 100μl of each standard, sample, control and zero (Standard Diluent) in duplicate to appropriate number of wells
  2. Add 50μl of diluted biotinylated anti-CD117 to all wells
  3. Cover with a plastic plate cover and incubate at room temperature (18 to 25°C) for 1 hour
  4. Remove the cover and wash the plate
  5. Add 100μl of Streptavidin-HRP solution into all wells
  6. Cover with a plastic plate cover and incubate at room temperature (18 to 25°C) for 30 min
  7. Repeat wash step 4.
  8. Add 100μl of ready-to-use TMB Substrate Solution into all wells
  9. Incubate in the dark for 10-20 minutes* at room temperature. Avoid direct exposure to light by wrapping the plate in aluminium foil
  10. Add 100μl of H2SO4:Stop Reagent into all wells

Typical Standard Curve

Manual

Product Manual