Human Artemin ELISA Assay Kit

$895.00

The Eagle Biosciences Human Artemin ELISA Assay Kit is intended for the quantitative determination of hARTN in serum, plasma, and cell culture supernatant. The Human Artemin ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.  The kit has been developed based on unique Artemin antibodies, which are raised against full-length recombinant protein expressed in mammalian cell culture. As is the case with many antibodies, they have high affinity and specificity (no cross reactivity to other neural growth factors).

SKU: ART31-K01 Categories: , ,

Human Artemin ELISA Assay Kit:

For Research Use Only

Size: 1×96 wells
Sensitivity: 2 pg/mL
Dynamic Range: 30 – 1920 pg/mL
Incubation Time: 2 hours
Sample Type: Serum, Plasma, Cell Culture Supernatant
Sample Size: 100 µL

Additional Information

Assay Principle

Eagle Biosciences hARTN (Human Artemin) test is based on the quantitative sandwich enzyme immunoassay technique. Microtiter wells are pre-coated with hARTN-specific monoclonal capture antibodies. Samples and standards are pipetted into microwells and hARTN molecules present in the sample are bound by the capture antibodies. After incubation, unbound material is removed by washing the wells. Then, horseradish peroxidase (HRP) conjugated hARTN-specific monoclonal detection antibodies bind to the same epitope on another molecule of dimeric hARTN. After washing, the ready-to-use HRP substrate (TMB) is added to the wells. The intensity of the colour produced is directly proportional to the amount of hARTN in the sample. Colour development is then stopped by the addition of stop solution. Absorbance is measured at 450 nm.

Assay Procedure

  1. Dilute 50 mL wash concentrate with 450 mL of distilled water.
  2. Prepare the standards directly before the test by serial dilutions using the stock standard and the sample diluent (pink).
  3. Perform the dilution of each sample in the sample diluent directly before the test.
  4. Add 100 μL samples and standards into appropriate wells in duplicate.
  5. Incubate the covered microplate for 60 min at RT on a microwell plate shaker (300 rpm).
  6. Remove the liquid and wash the wells 4 times with 300 μL of washing solution.
  7. Add 100 μL of enzyme conjugate (blue) into each well.
  8. Incubate the covered microplate for 30-60 min at RT on a microwell plate shaker (300 rpm).
  9. Remove the liquid and wash the wells 4 times with 300 μL of washing solution.
  10. Add 100 μL of substrate solution into each well.
  11. Incubate the covered microplate for 20 minutes at RT on a microwell plate shaker (300 rpm).
  12. Stop the reaction by adding 50 μl of STOP solution into each well in the same order and time as for TMB distribution.
  13. Read the absorbance at 450 nm immediately.

Typical Standard Curve

Manual

Product Manual