HtrA4 ELISA Assay Kit
The HtrA4 ELISA Assay Kit is For Research Use Only
Size:1 x 96 wells
Sensitivity: 1 ng/mL
Dynamic Range: 1.25 ng/ml to 80 ng/ml
Incubation Time: 4.25 hours
Sample Type: Serum, tissue, cell culture supernatants
Sample Size: 100 µL
Controls Included
Assay Background
Human HtrA4, the newest member of the HtrA family, an unique family of multidomain serine proteases, was initially reported as a serine protease associated with pregnancy. Polypeptide chains of human HtrA4 consist of several domains: An N-terminal insulin like growth factor domain, followed by a Kazal-type serine protease inhibitor domain, a linker region, a trypsin-like protease domain and a PDZ domain. Two studies reported in 2012 an association between abnormal levels of HtrA4 and preeclamsia. However, the findings are contradictory, while the study by Inagaki et al. [3] reported an elevation of HtrA4 in preeclampsia, the study by Wang et al. published that HtrA4 expression was decreased in preeclampsia. Therefore, the precise association between HtrA4 and preeclampsia was for some years unclear, till recently, when Sing et al. [4] published new data that showed that human HtrA4 expression was restricted to the placenta and significantly up-regulated in early-onset but not late-onset preeclampsia. The serm HtrA4 levels in normal pregnancy increased significantly between the first and second trimesters, then remained constant. Women with early onset but not late-onset preeclampsia showed significantly higher HtrA4 levels in serum compared with gestational age-matched controls.
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