HistaSure Dipstick Assay Kit


The Eagle Biosciences HistaSure dipstick assay kit is intended for the rapid screening of histamine in different scombroid fish types such as tuna, mahi mahi, sardines and for the screening of histamine in fishmeal.

SKU: FC L-3200 Categories: ,

HistaSure Dipstick Assay Kit

For Research Use Only

Size: 24 dipsticks
Standard Range: Cut-off pre-set at 50 ppm – adjustable
Incubation Time: 10 min
Sample Type: fish and fish-derived products
Sample Size: 10g

Controls Included

Additional Information

Assay Background

Histamine testing in fish is a possible control strategy that can be used by seafood processors in their HACCP program to address the hazard of scombrotoxin formation. Histamine is a product of decomposition of histidine caused by the growth of certain bacteria in seafood. The amount of the amine that forms is a function of bacterial species, the temperature and time of exposure, and may exceed 1,000 ppm (mg/kg). Fish containing high levels of histamine have been associated with many instances of poisoning commonly referred to as “scombroid poisoning,” a major health problem for consumers. Scombrotoxic fish usually contain levels of histamine in excess of 200 ppm but such fish may be randomly dispersed within a lot. For large fish, histamine is found at variable levels even within individual fish.
Quality control measures designed to minimize the occurrence of scombrotoxic fish require the determination of histamine levels in the range of approximately 10 to 200 ppm. Good quality fish contain less than 10 ppm histamine, a level of 30 ppm indicates significant deterioration, and 50 ppm is considered to be evidence of definite decomposition. The defect action level (DAL), the level at which regulatory actions are taken for histamine is 50 ppm (P. L. Rogers, W. F. Staruszkiewicz, Journal of Aquatic Food Product Technology, Vol. 9 (2) 2000 p. 5 – 17.)

Assay Principle

After a simple and quick water extraction step, the histamine in the sample is quantitatively derivatized into N-acylhistamine. After dilution of the N-acylated histamine in running buffer the Lateral flow device is added to the sample. The amount of immunogold labelled antibody bound to the solid phase histamine is inversely proportional to the histamine concentration in the sample.


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