HGH ELISA Assay Kit

$230.00

The Eagle Biosciences HGH ELISA Assay kit is a direct solid phase enzyme immunoassay for the quantitative measurement of Growth Hormone in human serum or plasma. The HGH ELISA Assay kit is intended for research use only.

SKU: DCM050 Categories: , ,

HGH ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.105 mIU/ml
Dynamic Range: 2 – 150 µIU/ml
Incubation Time: 1.5 hour
Sample Type: Serum, Plasma
Sample Size: 50 µL

Controls Included

Product Developed and Manufactured in Italy by Diametra

Additional Information

Assay Background

Growth hormone is a polypeptide hormone synthesised and secreted by the anterior pituitary gland which stimulates growth and cell reproduction in humans and other vertebrate animals.  GH is released from the pituitary into the bloodstream in a pulsatile manner under the regulatory control of hypothalamic somatostatin (SS) and GH-releasing factor (GHRF).  Stimulators of GH secretion include, exercise, hypoglycemia, dietary protein, and estradiol. Inhibitors of GH secretion include dietary carbhoydrate and glucocorticoids.

Almost 50% of GH in circulation is bound to a high affinity GH binding protein (GHBP), which represents the extracellular domain of the GH receptor.  The plasma concentration of GH during these peaks may range from 5 to 30 ng/mL or more. Peaks typically last from 10 to 30 minutes before returning to basal levels. The largest and most predictable of these GH peaks occurs about an hour after onset of sleep. Otherwise there is wide variation between days and individuals. Between the peaks, basal GH levels are low, usually less than 3 ng/mL for most of the day and night.

The amount and pattern of GH secretion change throughout life. Basal levels are highest in early childhood. The amplitude and frequency of peaks is greatest during the pubertal growth. Healthy children and adolescents average about 8 peaks per 24 hours. Adults average about 5 peaks. Basal levels and the frequency and amplitude of peaks decline throughout adult life.

The primary biological actions of the hormone are in direct growth promoting. HGH exerts its effect directly on target organs such as cartilage, bones and muscles and indirectly through the release of insulin-like growth factor (IGF), produced in the liver (2). In particular, somatotropin C (IGF-1) is essential for bone growth during childhood.

Generally increases protein synthesis and stimulates the growth of all internal organs excluding the brain. GH reduces liver uptake of glucose, an effect that opposes that of insulin. GH also contributes to the maintenance and function of pancreatic islets. GH stimulates the immune system. GH can cause excessive growth, traditionally referred to as pituitary giganttism.

Deficiency of GH produces significantly different problems at various ages. In children, growth failure and short stature are the major manifestations of GH deficiency. In adults the effects of deficiency are more subtle, and may include deficiencies of strength, energy, and bone mass, as well as increased cardiovascular risk.

Assay Principle

In this HGH ELISA Assay Kit, the calibrators and the patient specimens and/or controls (containing the native HGH antigen) are first added to streptavidin coated wells. Biotinylated monoclonal and enzyme labelled antibodies are then added: these antibodies have high affinity and specificity and are directed against distinct and different epitopes of HGH.

Reaction between the various HGH antibodies and native HGH occurs in the microwells without competition or steric hindrance, forming a soluble sandwich complex.

After equilibrium is attained, the antibody-bound fraction is separated from unbound antigen by decantation or aspiration.  Then the activity of the enzyme HRP present on the surface of the well is quantified by reaction with the TMB Substrate to produce color.  The enzyme activity in the antibody-bound fraction is directly proportional to the native antigen concentration.  By utilizing several different serum references of known antigen values, a dose response curve is generated from which the antigen concentration of an unknown is ascertained.

Manual

Product Manual