Folic Acid Immunoaffinity Column
The Folic Acid Immunoaffinity Column is For Research Use Only
Size: 3ml, 10 columns
Many methods of Folic Acid determination based on HPLC-UV (High Performance Liquid Chromotography-Ultraviolet) or HPLC-FLD (High Performance Liquid Chromatography with Postcolumn Fluorescence Derivatization) detection show low selectivity if problematic matrices are applied. This method of determination of Folic Acid combines the high selectivity of IAC (compared to other separation materials (e.g. SAX)) with its potential to concentrate elute through use of purification by a HPLC column.
Folic Acid samples are to be extracted and analyzed with the method of E.S. Osseyi et al. [E.S. Osseyi, R.L. Wehling, J.A. Albrecht J. Chromatogr. A 1998; 826:235-240], e.g. vitamin tablets, liquid vitamin preparations, cell culture extracts. Example: 2g vitamin containing tablets are dissolved in 50ml PBS. The resulting extract may be filtered through a 0.45µm membrane filter.