FGF23 (C-Terminal) ELISA Assay Kit

$620.00

The Eagle Biosciences FGF23 (Fibroblast Growth Factor 23) ELISA Assay Kit is intended for the quantitative determination of FGF23 in serum or plasma. The Eagle Biosciences FGF23 (Fibroblast Growth Factor 23) ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.

FGF23 (C-Terminal) ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.1 pmol/L
Dynamic Range: 0.2 – 20 pmol/L
Incubation Time: overnight
Sample Type: serum, citrate plasma, EDTA plasma or heparin plasma
Sample Size: 50 µL
Species: Human
Controls Included

Product Developed and Manufactured by Biomedica


NOT FOR SALE IN THE US

Additional Information

Specificity

This Eagle Biosciences FGF23 (Fibroblast Growth Factor 23) ELISA Assay Kit recognizes endogenous and recombinant human FGF23. The assay measures both intact and C-terminal fragments of FGF23 and validated for both serum and plasma according to ICH Q2. This assay demonstrates good correlation with existing ELISA methods.

Assay Principle

  1. All reagents and samples must be at room temperature (18-26°C) before use in the FGF23 ELISA Assay Kit.
  2. Mark position for BLANK/STD/SAMPLE/CTRL (Blank/Standard/Sample/Control) on the protocol sheet.
  3. Take microtiter strips out of the bag, take a minimum of one well as Blank. Store unused strips with desiccant at 2-8°C in the bag. Strips are stable until expiry date stated on the label.
  4. Pipette 50 µl STD/SAMPLE/CTRL (Standard/Sample/Control) in duplicate into respective well.
  5. Add 50 µl AB (biotinylated anti FGF23 antibody, green cap, green dye) into each well.
  6. Cover tightly and incubate over night (20-24 h) at room temperature (18-24°C).
  7. Aspirate and wash wells 5x with 300 µl diluted WASHBUF (Wash buffer, natural cap). Remove remaining WASHBUF by strongly tapping plate against paper towel after the last wash.
  8. Add 100 µl CONJ (Conjugate, amber cap) into each well.
  9. Cover tightly and incubate for 1 hour at room temperature (18-24°C) in the dark.
  10. Aspirate and wash wells 5x with 300 µl diluted WASHBUF (Wash buffer, natural cap). Remove remaining
  11. WASHBUF by strongly tapping plate against paper towel after the last wash.
  12. Add 100 µl SUB (Substrate, blue cap) into each well.
  13. Incubate for 30 minutes at room temperature (18-24°C) in the dark.
  14. Add 50 µl STOP (Stop solution, white cap) into each well.
  15. Measure absorbance immediately at 450 nm with reference 630 nm, if available.

fgf23-c-terminal-elisa-assay-correlation-data

Typical Standard Curve

fgf23-c-terminal-elisa-assay-typical standard-curve

Conversion factor: FGF23, C-terminal: 1 pg/ml = 0.133 pmol/l (MW: 7.52 kDa)


Manual

Product Manual


Publications

References

  • The Use of Fibroblast Growth Factor 23 Testing in Patients with Kidney Disease. ER Smith, Clin J Am Soc Nephrol 2014; 9: 1283-1303.
  • Management of hyperphosphataemia in chronic kidney disease – challenges and solutions. Ketteler M et al., Clinical Kidney Journal, 2013; 6: 128-136.
  • Regulation of serum phosphate. E Lederer, J Physiol 2014; 592: 3985-3995.
  • Fibroblast growth factor 23. Smith ER et al., Annals of Clin Biochemistr 2014; 51: 203-227.

Reference List

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