Estradiol Immunoaffinity HPLC Column
The Estradiol Immunoaffinity HPLC Column is For Research Use Only
Size: 3ml, 10 columns
Many methods of Estrogens determination based on HPLC-UV detection show low selectivity if problematic matrices are applied. This method of content determination of Estrogens combines the high selectivity of immunoaffinity columns with its high potential to concentrate elute and of purification by HPLC column. The following instruction gives an optimized protocol for enrichment of very low contents in the pg/g range. In this case a mass detector might be best suited.
Please notice, that this instruction focuses on the handling with the IAC column. The given apparatus and detection (e.g. HPLC-FLD system) might serve as example among other possibilities.
Sewage and drinking water samples are collected as described by Quintana et al1. Raw samples may be filtered through a 0.45μm pore size cellulose filter. Example: 1000g sewage effluent sample (equals approximately 1L) firstly are filtered like mentioned before and then added to the filtrate 10ml Buffer Solution (see under Buffer and Chemicals) to maintain pH neutrality. Thus, the final sample contains 1% (v/v) Buffer Solution.