Endostatin Mouse/Rat ELISA
Endostatin Mouse Rat ELISA Developed and Manufactured in Austria by Biomedica
Size: 12×8 tests
Sensitivity: 0.24 nmol/L
Dynamic Range: 1-32 nmol/l
Incubation Time: 2.5 hours
Sample Type: Mouse or rat serum, plasma
Sample Size: 5 µL
Alternative Names: Rodent Endostatin ELISA
For Research Use Only
Conversion Factor: 1 ng/ml = 0.049 nmol/l or 1 nmol/l=20.376 ng/ml (MW: 20.4 kDa)
Specificity: Endogenous and recombinant mouse and rat Endostatin.
Mouse sera C57BL6JOlaHsd, 12 weeks (n=11): 6.7 ± 0.8 nmol/l
Wildtype normal mice sera, 12 weeks, male (n=10): 5.4 ± 1.2 nmol/l
Wildtype normal rat sera, 12 weeks, male (n=8): 2.5 ± 0.4 nmol/l
Each laboratory should establish its own reference range for the samples under investigation.
The Endostatin Mouse/Rat ELISA kit is a sandwich enzyme immunoassay for the direct determination of endostatin in mouse/rat serum and plasma samples. In a first step, 1+50 pre-diluted STD/sample/CTRL and conjugate (goat anti-endostatin-HRPO) are pipetted into the wells of the microtiter strips, which are pre-coated with anti mouse endostatin antibody. Endostatin present in the STD/sample/CTRL binds to the pre-coated antibody in the well and forms a sandwich with the detection antibody in the conjugate. In the washing step all non-specific unbound material is removed. In a second step, the substrate (TMB Tetramethylbenzidine) is pipetted into the wells. The enzyme catalysed colour change of the substrate is directly proportional to the amount of endostatin. This colour change is detectable with a standard microtiter plate ELISA reader. A dose response curve of the absorbance (optical density, OD at 450 nm) vs. standard concentration is generated, using the values obtained from the standard. The concentration of mouse/rat endostatin in the sample is determined directly from the dose response curve.