Endostatin Mouse Rat ELISA

$645.00

The Endostatin Mouse/Rat ELISA Assay is intended for the quantitative determination of mouse/rat endostatin in serum and plasma.For Research Use Only.

SKU: BI-20742-MR Categories: , ,

Endostatin Mouse/Rat ELISA

Endostatin Mouse Rat ELISA Developed and Manufactured in Austria by Biomedica

Size: 12×8 tests
Sensitivity: 0.24 nmol/L
Dynamic Range: 1-32 nmol/l
Incubation Time: 2.5 hours
Sample Type: Mouse or rat serum, plasma
Sample Size: 5 µL
Alternative Names: Rodent Endostatin ELISA
For Research Use Only

Controls Included

Conversion Factor: 1 ng/ml = 0.049 nmol/l or 1 nmol/l=20.376 ng/ml (MW: 20.4 kDa)
Specificity: Endogenous and recombinant mouse and rat Endostatin.
Reference Range:
Mouse sera C57BL6JOlaHsd, 12 weeks (n=11): 6.7 ± 0.8 nmol/l
Wildtype normal mice sera, 12 weeks, male (n=10): 5.4 ± 1.2 nmol/l
Wildtype normal rat sera, 12 weeks, male (n=8): 2.5 ± 0.4 nmol/l
Each laboratory should establish its own reference range for the samples under investigation.

Assay Principle

The Endostatin Mouse/Rat ELISA kit is a sandwich enzyme immunoassay for the direct determination of endostatin in mouse/rat serum and plasma samples. In a first step, 1+50 pre-diluted STD/sample/CTRL and conjugate (goat anti-endostatin-HRPO) are pipetted into the wells of the microtiter strips, which are pre-coated with anti mouse endostatin antibody. Endostatin present in the STD/sample/CTRL binds to the pre-coated antibody in the well and forms a sandwich with the detection antibody in the conjugate. In the washing step all non-specific unbound material is removed. In a second step, the substrate (TMB Tetramethylbenzidine) is pipetted into the wells. The enzyme catalysed colour change of the substrate is directly proportional to the amount of endostatin. This colour change is detectable with a standard microtiter plate ELISA reader. A dose response curve of the absorbance (optical density, OD at 450 nm) vs. standard concentration is generated, using the values obtained from the standard. The concentration of mouse/rat endostatin in the sample is determined directly from the dose response curve.

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Additional Information

Assay Background

Endostatin, a 20-kDa C-terminal proteolytic fragment of collagen XVIII, is an endogenous angiogenesis inhibitor localized in the vascular basement membrane in various organs (http://www.uniprot.org/uniprot/P39060). The biological functions of the endostatin-network involve SPARC, thrombospondin-1, glycosaminoglycans, collagens, and integrins. In animal studies, renal Endostatin expression preceded deteriorating kidney function and induced renal fibrosis in aging mice. In humans, Endostatin is expressed during the progression of renal fibrosis in tubular cells of injured tissue. In renal micro-vascular disease, observed in late stages of patients with chronic kidney disease, increased endostatin levels are possibly the consequence of enhanced extracellular matrix degradation. Thus endostatin may become an important marker for progressive microvascular renal disease in patients with chronic kidney disease. Endostatin levels in blood are also likely to increase in patients with other microvascular tissue injuries, including atherosclerosis, myocardial- and brain ischemia. In ischemic stroke patients, high endostatin plasma levels predict a worse long-term clinical outcome. In a cohort of critically ill patients, plasma endostatin improved AKI prediction based on clinical risk factors. Endostatin has evolved as a molecular target and is currently under investigation in clinical trials.

Manual

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