ENA screen ELISA Kit
ENA screen ELISA Kit Developed and Manufactured by Medipan
Size: 1×96 wells
Sensitivity: Cut-off Controls
Incubation Time: 2 hours
Sample Type: Serum, Plasma
Alternative Names: Extractable Nuclear Antigen screen ELISA, Human ENA screen ELISA, Human Extractable Nuclear Antigen screen ELISA
Sample Size: 100 µL
For Research Use Only
Positive: ≥ 1.0
Negative: < 1.0 It is recommended that each laboratory establishes its own normal and pathological reference ranges, as usually done for other diagnostic parameters, too. Therefore, the above mentioned reference values provide a guide only to values which might be expected. Sensitivity
The analytical sensitivity of each readivity of the ENA screen (Extractable Nuclear Antigen) ELISA is around 0.2.
ENA screen (Extractable Nuclear Antigen) ELISA assay kit is an enzyme immunoassay for the semi-quantitative determination of IgG antibodies to nuclear and cytoplasmic antigens. Antibodies of the calibrator and diluted samples react with nuclear and cytoplasmic antigens immobilized on the solid phase of microtiter plates. Recombinant SS-B, Sm, RNP (68 kDa, A, C), Scl-70, Jo-1 as well as highly purified Sm and SS-A guarantee the specific binding of autoimmune antibodies of the specimen under investigation. Following an incubation period of 60 min at room temperature (RT), unbound sample components are removed by a wash step.
The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish peroxidase (HRP). Within the incubation period of 30 min at RT, excessive conjugate is separated from the solid-phase immune complexes by the following wash step. HRP converts the colorless substrate solution of 3,3’,5,5’-tetramethylbenzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution into the wells after 15 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. The cut-off is established by multiplying the OD of the calibrator with the corresponding factor.