Dopamine & Noradrenaline Sensitive ELISA
Dopamine & Noradrenaline Sensitive ELISA is manufactured in Germany as SKU EA631/192 by DLD Diagnostika
Size: 2×96 wells (1 plate for Dopamine, 1 plate for Noradrenaline)
Sensitivity: Dopamine: 4.9 pg/ml, Noradrenaline: 2.4 pg/ml
Dynamic Range: Dopamine: 0.3 – 100 ng/ml, Noradrenaline: 0.15 – 25 ng/ml
Incubation Time: Overnight
Sample Type: Biological Samples
Sample Size: 20 µl
Alternative Names: Catecholamine ELISA Kit, Dopamine & Norepineprhine ELISA Kit
For Research Use Only
Controls Included
Conversion into pmol/l:
Noradrenaline: 1 pg / ml = 5,91 pmol/l
Dopamine: 1 pg / ml = 6,53 pmol/l
Assay Principle for Dopamine and Noradrenaline ELISA
The Dopamine & Noradrenaline High Sensitive ELISA is competitive and uses the microtiter plate format. Dopamine and Noradrenaline, respectively are bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG/peroxidase. The substrate TMB/peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.
Related Products
Adrenaline, Noradrenaline & Dopamine ELISA Assay Kit
Dopamine Sensitive ELISA Assay Kit
Noradrenaline (Norepinephrine) Sensitive ELISA