Additional Information

Assay Background

The Dopamine & Noradrenaline High Sensitive ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of noradrenaline in biological samples including serum, plasma, tissue, and cell culture samples. The Dopamine & Noradrenaline High Sensitive ELISA Assay kit is for research use only and should not be used in diagnostic procedures. Catecholamine is the name of a group of aromatic amines (noradrenaline, adrenaline, dopamine, and their derivatives) which act as hormones and neurotransmitter, respectively. Adrenaline and noradrenaline are formed from dopamine. They act on the cardiac musculature and the metabolism (adrenaline) as well as on the peripheral circulation (noradrenaline) and help the body to cope with acute and chronic stress.

An increased production of catecholamines can be found with tumours of the chromaffine system (pheochromocytoma, neuroblastoma, ganglio­neuroma). An increased or decreased concentration of the catechol­amines can also be found with hypertension, degenerative cardiac diseases, schizophrenia and manic-depressive psychosis. The Dopamine & Noradrenaline High Sensitive ELISA Assay kit provides materials for the quantitative measurement of noradrenaline and dopamine in low concentrated samples and for small sample volumes. Noradrenaline and dopamine are extracted using a cis-diol-specific affinity gel and acylated to N-acylnoradrenaline and N-acyldopamine and then converted enzymatically into N-acylnormeta­nephrine and N-acyl-3-methoxytyramine.

Assay Principle

The competitive Dopamine & Noradrenaline High Sensitive ELISA Assay kit uses the microtiter plate format. Noradrenaline and dopamine, respectively, are bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG/peroxidase. The substrate TMB/peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.

Typical Standard Curve