Diaminooxidase (DAO) REA (³H-Nuclid) ELISA Assay Kit


The Eagle Biosciences Diaminooxidase (DAO) REA (³H-Nuclid) ELISA Assay Kit is intended for the quantitative determination of diamine oxicdase activity in serum and plasma. It is for research use only. Not for use in diagnostic procedures.

SKU: KR8220 Categories: , ,

Diaminooxidase (DAO) REA (³H-Nuclid) ELISA Assay Kit

For Research Use Only

Size: 100 tests
Standard Range: 2.1 – 80 U/ml
Incubation Time: 2.5 h
Sample Type: Serum
Sample Size: 100 µl

Product manufactured in Germany by Immundiagnostik

Additional Information

Assay Background

Diamine oxidase (DAO) is a body‘s own enzyme that metabolizes histamine. Although DAO is found practically in the whole body, the most important site of its action is the intestine. The enzymatic activity of DAO determines the histamine degradation speed. In the case of DAO deficiency or inhibition, incorporated or endogenous histamine cannot be degraded quickly enough, and the symptoms of histamine intolerance are presented. Millions of people suffer from gastrointestinal problems, migraine, irritations of nasal mucosa and other allergy-like symptoms after consumption of certain nutrients. Too much histamine in the body can be the reason for this wide range of symptoms. Another possibility for reduced DAO function could be the intake of activity-inhibiting substances, such as alcohol or medication. Histamine induced food intolerance is not IgE-mediated. Determination of the DAO activity in serum or plasma is a suitable marker for diagnosis of histamine intolerance and the associated symptoms. With our easy-to-use, reliable and standardised test kit it is possible to quantify the biological activity of DAO in the circulation. Only 100µl of serum is needed for the test, results are available within 3 hours.

Assay Principle

Human serum may be used as an analyte. The activity of the diamine oxidase is determined by quantitating the reaction product. Radiolabelled putrescine-dihydrochloride is used as a substrate. The resulting 1 pyrroline, containing the radiolabel, is extracted selectively from the matrix by a liquid extraction step. A non-toxic, chlorine free solvent with high capacity is used for the extraction. Finally szintillation fluid is added to the organic phase containing the radiolabelled 1 pyrroline and radioactivity is determined in a beta-counter. The signal is directly proportional to the activity of DAO in the sample.


Product Manual