Deoxynivalenol ELISA Assay Kit
The Deoxynivalenol ELISA Assay Kit is For Research Use Only
Size: 12×8 wells
Sensitivity: 12 ppb
Dynamic Range: 3.07 to 120 ng/ml
Incubation Time: 0.75 hours
Sample Type: Food and Feed Crop Probes
The Deoxynivalenol ELISA is a competitive enzyme immunoassay. The assay is carried out in a microplate, of which the cavities have already been pre-coated with a special anti- Deoxynivalenol antibody.
To carry out the assay, Deoxynivalenol (DON) standards and sample extracts respectively are pipetted into the cavities. After 15 min incubation the Deoxynivalenol peroxidase conjugate (DON-HRP-conjugate) is added without a preceding washing step.
Deoxynivalenol from the standard or sample competes with the HRP labelled Deoxynivalenol for the antibody binding sites. The unbound components are removed by rinsing them off and then peroxidase substrate solution (3,3’,5,5’-Tetramethylbenzidine, TMB) is pipetted into all cavities. The DON-HRP conjugate that is bound to the antibody reacts with the substrate solution by forming a blue colour. After 15 minutes, this reaction is terminated by adding the stop solution. The color then changes from blue to yellow, which is detected photometrically. With the help of a microplate reader, the yellow coloring is measured as an optical density (OD) at a wavelength of 450nm (reference value 620nm).
The Deoxynivalenol concentration is inversely proportional to the color intensity. The higher the OD measurement, the lower is the concentration of Deoxynivalenol in the standard or sample.