Deoxynivalenol ELISA Assay Kit

$305.00

The Eagle Biosciences Deoxynivalenol ELISA Assay Kit allows the quantitative determination of Deoxynivalenol in food and feed crop (cereals). For further applications, the user is encouraged to verify the validity of the tests themselves or contact the manufacturer.

The test is easy to use, requires no complex instrumental equipment, and delivers the sensitive results quickly.  After a sample preparation to isolate the Deoxynivalenol, a maximum of 36 samples in duplicate can be tested in 45 minutes with the Deoxynivalenol  ELISA. The sample extraction with water is described in the following. Alternatively, Deoxynivalenol can also be isolated with the help of immunoaffinity chromatography. The Eagle Biosciences Deoxynivalenol ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: BTDOEK-001 Categories: , ,

Deoxynivalenol ELISA Assay Kit

For Research Use Only

Size: 12×8 wells
Sensitivity:   12 ppb
Dynamic Range:  3.07 to 120 ng/ml
Incubation Time: 0.75 hours
Sample Type: Food and Feed Crop Probes


Additional Information

Assay Background


Deoxynivalenol (DON), also known as Vomitoxin, is a type of mycotoxin (toxic mould fungus). Chemically it belongs to type B trichothecenes. The mycotoxin DON is formed as a poisonous metabolite from the Fusarium genus, i.e. F. culmorum and  F. graminearum.

DON enters into the human food chain via contamination of plant-based foods. It is abundant in grains such as wheat, barley, oats, rye, maize and processed grains such as malt, beer and bread. The main toxin production by Fusarium species happens in the field. Climate conditions have a big influence on contamination.The harvest and storage conditions play also an important role in DON contamination

DON is a potent inhibitor of protein synthesis and thus acts generally cause cell damage.The mycotoxin DON has been connected with incidents of mycotoxicoses in both humans and farm animals

Assay Principle


The Deoxynivalenol ELISA is a competitive enzyme immunoassay. The assay is carried out in a microplate, of which the cavities have already been pre-coated with a special anti- Deoxynivalenol antibody.

To carry out the assay, Deoxynivalenol (DON) standards and sample extracts respectively are pipetted into the cavities. After 15 min incubation the Deoxynivalenol peroxidase conjugate (DON-HRP-conjugate) is added without a preceding washing step.

Deoxynivalenol from the standard or sample competes with the HRP labelled Deoxynivalenol for the antibody binding sites. The unbound components are removed by rinsing them off and then peroxidase substrate solution (3,3’,5,5’-Tetramethylbenzidine, TMB) is pipetted into all cavities. The DON-HRP conjugate that is bound to the antibody reacts with the substrate solution by forming a blue colour. After 15 minutes, this reaction is terminated by adding the stop solution. The color then changes from blue to yellow, which is detected photometrically. With the help of a microplate reader, the yellow coloring is measured as an optical density (OD) at a wavelength of 450nm (reference value 620nm).

The Deoxynivalenol concentration is inversely proportional to the color intensity. The higher the OD measurement, the lower is the concentration of Deoxynivalenol in the standard or sample.

Standard Curve


Manual

Product Manual