Cystatin C EIA Kit


The Cystatin C EIA Kit is designed to quantitatively measure human Cystatin C present in biological samples and tissue culture media.

SKU: SKT-219-96 Categories: , ,

Cystatin C EIA Kit

The Cystatin C EIA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.058 ng/ml
Dynamic Range: 0.156 – 10 ng/ml
Incubation Time: 2 hours
Sample Type: EDTA Plasma, Heparin Plasma, Serum, Tissue Culture Media, Urine
Sample Size: 50 μL

Product manufactured in Canada by StressMarq.

Assay Background

Cystatin C is a non-glycosylated protein of low molecular weight (13kDa) in the cystatin superfamily. Cystatin C is produced at a constant rate in all nucleated cells, secreted from cells and thus found in detectable amounts in most body fluids. Cystatin C belongs to the cysteine proteinase inhibitor group and is associated with several pathological conditions. Imbalance between Cystatin C and cysteine proteinases is associated with diseases such as inflammation, renal failure, cancer, Alzheimer’s disease, multiple sclerosis and hereditary Cystatin C amyloid angiopathy. Increased levels have been found in autoimmune diseases, with colorectal tumors and metastases, patients with inflammation and in patients on dialysis. Cystatin C is removed from blood plasma by glomerular filtration in the kidneys. It is reabsorbed by the proximal tubular cells and degraded. There is a linear relationship between the reciprocal Cystatin C concentration in plasma and the glomerular filtration rate (GFR). Cystatin C is suggested to be a better marker for GFR than the ubiquitous serum creatinine marker as its serum concentration is not affected by other factors such as age, gender and body mass and Cystatin C has higher sensitivity to detect a reduced GFR than creatinine determination. Low levels of Cystatin C are found with the breakdown of the elastic laminae and atherosclerosis and abdominal aortic aneurysm. There is evident association of Cystatin C levels with the incidence of myocardial infarction, coronary death and angina pectoris, presenting a risk factor for secondary cardiovascular events.

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Additional Information

Assay Principle

The Cystatin C EIA kit is designed to quantitatively measure human Cystatin C present in biological samples and tissue culture media. A human Cystatin C standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with a monoclonal antibody to capture the Cystatin C present. After a 60 minute incubation, the plate is washed and a peroxidase conjugated Cystatin C monoclonal antibody is added. The plate is again incubated for 30 minutes and washed. Substrate is then added to the plate, which reacts with the bound Cystatin C Antibody Conjugate. After a third incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the Cystatin C in the sample is calculated, after making suitable correction for dilution, using software available with most plate readers.

Typical Standard Curve

Cystatin C EIA Kit