Cortisone CLIA Kit

$490.00

The Cortisone CLIA Kit is designed to quantitatively measure Cortisone present in extracted dried fecal samples, urine, saliva, and serum samples.

SKU: SKT-206-96 Categories: , ,

Cortisone CLIA Kit

The Cortisone CLIA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 10.6 pg/ml
Dynamic Range: 78.1 – 20,000 pg/ml
Incubation Time: 2 hours
Sample Type: Dried Fecal Samples, Plasma, Saliva, Serum, Tissue Culture Media, Urine
Sample Size: 50 μL

Product manufactured in Canada by StressMarq.


Assay Background

Cortisone (C21H28O5, Kendall’s Compound ‘E’) was identified by Mason, Myers and Kendall in 1936 as Compound E extracted from bovine suprarenal gland tissue that had the qualitative but not quantitative activity of cortin. The presence of multiple cortin-like compounds led the authors to speculate that the study of Compound E would reveal the nature of cortin. Compound E is now called cortisone and the more active Compound F, cortisol, and the concentrations of these two glucocorticoids vary due to the activity of two 11ß-hydroxysteroid dehydrogenases (11-HSD). While most tissues have the ability to express either enzyme, 11ß-HSD1 is found primarily in the liver where it converts cortisone to cortisol while 11ß-HSD2 is found in tissues such as the kidney where cortisol receptor binding is required. 11ß-HSD2 deactivates cortisol to cortisone, prohibiting receptor activation. This glucocorticoid “shuttle” helps to initiate and regulate the anti-inflammatory response, making cortisone one of the modern “wonder drugs”. Monitoring the ratio of cortisone:cortisol has applications in diabetes, obesity, metabolic syndrome, osteoporosis, and chronic fatigue syndrome in addition to adrenal diseases. Cortisone and cortisol concentrations exhibit a predictable diurnal pattern and can be measured in extracted dried feces, or in serum, plasma, saliva and urine. A recent publication has suggested that salivary cortisone is a good surrogate marker for serum cortisol.


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Additional Information

Assay Principle


The Cortisone CLIA kit is designed to quantitatively measure Cortisone present in extracted dried fecal samples, urine, saliva, and serum samples. This kit measures total cortisone in serum and plasma and in extracted fecal samples. A cortisone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a white microtiter plate coated with an antibody to capture rabbit antibodies. A cortisone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to cortisone to each well. After a two hour incubation the plate is washed and the chemiluminescent substrate is added. The substrate reacts with the bound cortisone-peroxidase conjugate to produce light. The generated light is detected in a microtiter plate reader capable of reading luminescence. The concentration of the cortisone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Typical Standard Curve


Cortisone CLIA Kit

Manual

Product Manual