Cortisone CLIA Kit
The Cortisone CLIA Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 10.6 pg/ml
Dynamic Range: 78.1 – 20,000 pg/ml
Incubation Time: 2 hours
Sample Type: Dried Fecal Samples, Plasma, Saliva, Serum, Tissue Culture Media, Urine
Sample Size: 50 μL
Product manufactured in Canada by StressMarq.
Assay Background
Cortisone (C21H28O5, Kendall’s Compound ‘E’) was identified by Mason, Myers and Kendall in 1936 as Compound E extracted from bovine suprarenal gland tissue that had the qualitative but not quantitative activity of cortin. The presence of multiple cortin-like compounds led the authors to speculate that the study of Compound E would reveal the nature of cortin. Compound E is now called cortisone and the more active Compound F, cortisol, and the concentrations of these two glucocorticoids vary due to the activity of two 11ß-hydroxysteroid dehydrogenases (11-HSD). While most tissues have the ability to express either enzyme, 11ß-HSD1 is found primarily in the liver where it converts cortisone to cortisol while 11ß-HSD2 is found in tissues such as the kidney where cortisol receptor binding is required. 11ß-HSD2 deactivates cortisol to cortisone, prohibiting receptor activation. This glucocorticoid “shuttle” helps to initiate and regulate the anti-inflammatory response, making cortisone one of the modern “wonder drugs”. Monitoring the ratio of cortisone:cortisol has applications in diabetes, obesity, metabolic syndrome, osteoporosis, and chronic fatigue syndrome in addition to adrenal diseases. Cortisone and cortisol concentrations exhibit a predictable diurnal pattern and can be measured in extracted dried feces, or in serum, plasma, saliva and urine. A recent publication has suggested that salivary cortisone is a good surrogate marker for serum cortisol.
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