Citrulline ELISA Assay Kit

$650.00

The Eagle Biosciences Citrulline ELISA Assay Kit is for the quantitative determination of Citrulline in urine, serum, plasma and various biological samples.

SKU: BA E-2800 Categories: , ,

Citrulline ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.23 µg/ml
Standard Range: 0.6 – 60 µg/ml
Incubation Time: overnight, 2 x 30 min
Sample Type: Urine, serum, plasma
Sample Size: 50 µl

Controls Included

Additional Information

Assay Principle

The samples are first cleaned up by an extraction procedure. After derivatization Citrulline is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.

Manual

Product Manual