Canine IgG ELISA Assay Kit

$330.00

The Eagle Biosciences Canine IgG ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgG in biological samples of Dogs.

Canine IgG ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 7.148 ng/ml
Dynamic Range: 25 ng/ml – 800 ng/ml
Incubation Time: 40 minutes
Sample Type: Plasma, Serum
Sample Size: 100 μL

Product manufactured in the U.S.A

Additional Information

Assay Principle


The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Typical Standard Curve


Manual

Product Manual