Calcitonin ELISA Assay Kit


The Eagle Biosciences Cacitonin ELISA Assay Kit is an immunoenzymatic colorimetric method for quantitative determination of Calcitonin concentration in human serum. Calcitonin kit is intended for research use only and not for use in diagnostic procedures.

Calcitonin ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.7 pg/mL
Incubation Time: 1.5 hours
Sample Type: Serum
Sample Size: 100 µL

Controls Included

Product Developed and Manufactured in Italy by Diametra

Additional Information

Assay Principle

The Eagle Biosciences Calcitonin ELISA Assay Kit is based on the simultaneous capture of the antigen by two monoclonal antibodies (one coated on the microplate, the other linked to horseradish peroxidase) and subsequent quantification via a chromogenic substrate. In the first phase an incubation of calibrators, controls and patient samples in the microtiter plate with the conjugate reagent is carried out; antibodies to Calcitonin form a sandwich with the antigen, blocking Calcitonin in the wells of the microplate

After incubation, the microplate is washed with a washing solution for the removal of components that have not reacted. Finally, a chromogenic substrate solution containing TMB is added. After the incubation, the reaction is stopped by adding the Stop Solution. The color of the solution turns yellow. The amount of color developed is directly proportional to the concentration of Calcitonin in the sample. The concentration of Calcitonin in the sample is calculated through a calibration curve.

Assay Background

Calcitonin is a hormone secreted by the parafollicular C cells of the thyroid gland, that acts on calcium levels by modulating the ability to “clearance” of renal calcium and reducing bone resorption. It is unclear, however, the physiological result of this modulation. An excess of Calcitonin in the blood tissue may be indicative of the onset of malignant tumor “medullary thyroid cancer” (MTC), that mainly (80% of cases) appears sporadically, but that also has a familial component (20% of cases).

A moderate increase of Calcitonin in the blood can be found in the states of pregnancy, pernicious anemia, renal dysfunction, and during the neonatal period. Therefore, the quantification of Calcitonin in the blood tissue can be useful mainly in the diagnosis and follow-up of MTC, but also in the study of the pathophysiology of calcium and bone metabolism.


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