Bovine IgG ELISA


The Eagle Biosciences Bovine IgG ELISA Assay Kit is designed for the quantitative determination of IgG antibodies in bovine serum and biological samples. The Bovine IgG ELISA Assay Kit is for research use only and should not be used in diagnostic procedures. Main application of the assay is to monitor purification of protein preparations (e.g. monoclonal antibodies) if bovine or calf serum has been used in the production process.

Bovine IgG ELISA

For Research Use Only

Size: 1×96 wells
Sensitivity: 15.6 ng/ml
Dynamic Range: 3.125 – 200 ng/ml
Incubation Time: 2.5 hours
Sample Type: Serum, Biological Fluids
Sample Size: 10 µL

Additional Information

Assay Principle

The determination of bovine IgG is carried out as direct sandwich ELISA. An antibody specific for bovine IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG con­cen­tration.

Assay Procedure

A. Preparation

  1. Bring all reagents to room temperature
  2. Dilute wash buffer 1:10
  3. Prepare the standard curve from a 1:2-dilution series of standard concentrate with diluent
  4. Dilute samples with diluent
  5. Dilute freshly HRP conjugate 1:100 with diluent

B. Performance

  1. Pipette 100 µl of samples, standards, controls into the wells
  2. Incubate for 1 hour at room temperature with shaking
  3. Wash three times with 300 µl of wash buffer
  4. Add 100 µl of HRP conjugate to each well
  5. Incubate for 1 hour at room temperature with shaking
  6. Wash three times with 300 µl of wash buffer
  7. Dispense 100 ml of TMB substrate solution
  8. Incubate for 15 minutes at room temperature in the dark
  9. Add 100 µl of stop solution
  10. Measure absorption at 450 nm

Typical Standard Curve


Product Manual