$435.00
The Eagle Biosciences Bovine (Cow) ITIH4 ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring ITIH4 in biological fluid of Bovines.
For Research Use Only
Size: 1×96 wells
Sensitivity: 4.001 ng/ml
Dynamic Range: 6.25 ng/ml – 200 ng/ml
Incubation Time: 130 minutes
Sample Type: Plasma, Serum
Sample Size: 100 μL
Product manufactured in the U.S.A
The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the ITIH4 present in samples reacts with the anti- ITIH4 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-ITIH4 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound ITIH4. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of ITIH4 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of ITIH4 in the test sample. The quantity of ITIH4 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
