BNP Fragment (Nt-proBNP 8-29) ELISA Assay Kit

$600.00

The Eagle Biosciences BNP Fragment ELISA Assay Kit is intended for the quantitative determination of human BNP Fragment (Nt-proBNP 8-29) in serum, citrate plasma, EDTA plasma or heparin plasma. The BNP Fragment ELISA Assay Kit is manufactured by Biomedica (www.bmgrp.com).  The BNP Fragment ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: BI-20852W Categories: , ,

BNP Fragment (Nt-proBNP 8-29) ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 171 pmol/L
Dynamic Range: 200 – 6400 pmol/L
Incubation Time: Overnight
Sample Type: serum, citrate plasma, EDTA plasma or heparin plasma
Sample Size: 30 µL
Species: Human

Controls Included

Product Developed and Manufactured in Austria by Biomedica

Additional Information

Assay Background

The BNP Fragment (Nt-proBNP 8-29) ELISA Assay kit is an imunoassay for the quantitative determination of nt-proBNP (8-29) in Serum or Plasma. The mature form of brain natriuretic peptide (BNP) is formed from the BNP (1-108) precursor and is secreted predominantly by the cardiac ventricles. Circulating forms of BNP include BNP-32 (77-108), a high molecular weight BNP which has not been well characterizedand and the nt-proBNP (1-76). The BNP fragment is typically measured as a proxy for BNP because of its longer half-life. Recent research suggests that natriuretic peptides are important protectors against fluid overload and high blood pressure. BNP has also been implicated as a valuable marker in assessing cardiac risk.
The natriuretic peptides are members of a family of structurally similar but genetically distinct peptide hormones, consisting of atrial-, brain-, and C-type (ANP, BNP, and CNP, respectively). ANP and BNP preferentially bind to a membrane-bound guanylyl cyclase (GC) receptor called GC-A or NPR1, whereas CNP is the physiological ligand for GC-B (NPR2). The natriuretic peptides play an important role in the regulation of cardiovascular and renal homeostasis and in the regulation of fatty acid metabolism and body weight.
BNP is mainly expressed by ventricular myocardium in response to volume overload and increased filling pressure. BNP has a cleavable signal sequence. Mature BNP consists of 108 amino acids (proBNP or BNP-108), and undergoes cleavage resulting in physiologically active BNP-32 and additional C-terminal fragments along with a physiologically inactive N-terminal peptide comprising amino acids 1-76, which is further degraded proteolytically. BNP fragments in the circulation are therefore very heterogenous.
BNP has a key role in cardiovascular homeostasis with biological actions including natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. A high concentration of BNP in the bloodstream is indicative of heart failure. The discovery of natriuretic peptides identified an endocrine system that contributes to diuresis and vascular tone. The biology, biochemistry and the pathophysiological role of natriuretic peptides are described in several reviews.

Assay Principle

  • All reagents and samples must be at room temperature (18-26°C) before use in the BNP Fragment ELISA Assay Kit.
  • Mark position for BLANK/STD/SAMPLE/CTRL (Blank/Standard/Sample/Control) on the protocol sheet.
  • Take microtiter strips out of the bag, take a minimum of one well as Blank. Store unused strips with desiccant at 2-8°C in the bag. Strips are stable until expiry date stated on the label.
  1. Add 150 µl ASYBUF (Assay buffer) into all wells, except blank.
  2. Add 30 µl STD/ SAMPLE/ CTRL (Standards/Sample/Control) in duplicate into respective well, except blank.
  3. Add 50 µl CONJ (Conjugate) into each well, except blank, swirl gently.
  4. Cover tightly and incubate overnight (16-25 hours) at 4°C (2-8°C) in the dark.
  5. Aspirate and wash wells 5x with 300 µl diluted WASHBUF (Wash buffer), remove remaining WASHBUF by hitting plate against paper towel after the last wash.
  6. Add 200 µl SUB (Substrate) into each well.
  7. Incubate for 20 min at room temperature (18-26°C) in the dark.
  8. Add 50 µl STOP (Stop solution) into each well, shake well.
  9. Measure absorbance immediately at 450 nm with reference 630 nm, if available

Typical Standard Curve

Manual

Product Manual


Publications

References

View PDF

  • Thirty years of the heart as an endocrine organ: physiological role and clinical utility of cardiac natriuretic hormones.
    Clerico A et al., Am J Physiol Heart Circ Physiol 2011; 301: H12-H20
  • Comparison of Pleural Fluid N-Terminal Pro-Brain Natriuretic Peptide and Brain Natriuretic-32 Peptide Levels.
    Long AC et al., Chest 2010; 137: 1369-1374
  • N-Terminal Pro-B-Type Natriuretic Peptide as an Indicator of Possible Cardiovascular Disease in Severely Obese Individuals: Comparison with Patients in Different Stages of Heart Failure. Hermann-Arnhof K et al., Clinical Chemistry 2005; 51:138-143
  • Natriuretic Peptides: New Players in Energy Homeostasis.  Moro C and Smith RH, Diabetes 2009; 58: 27-26
  • Risk assessment in patients with unstable angina/non-ST-elevation myocardial infarction and normal N-terminal pro-brain natriuretic peptide levels by N-terminal pro-atrial natriuretic peptide. Jarai R et al., Eur Heart J 2004; 26: 250-256
  • Natriuretic peptides: Markers or modulators of cardiac hypertrophy? Gardner DG, Trends Endocrinol Metab 2003; 14: 411-416
  • Neurohormonal risk stratification for sudden death and death owing to progressive heart failure in chronic heart failure. Berger R et al, Eur J Clin Invest 2005, 35, 24-31
  • Natriuretic peptides/cGMP/cGMP-dependent protein kinase cascades promote muscle mitochondrial biogenesis and prevent obesity. Miyashita K et al., Diabetes 2009; 58: 2880-2892
  • Processing of Pro–B-Type Natriuretic Peptide: Furin and Corin as Candidate Convertases. Semenov AG et al., Clin Chem. 2010; 56:1166-1176

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